Transcription Factor Protocols
| Corporate Author: | |
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| Other Authors: | |
| Format: | eBook |
| Language: | English |
| Published: |
Totowa, NJ :
Humana Press : Imprint: Humana,
2000.
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| Edition: | 1st ed. 2000. |
| Series: | Methods in Molecular Biology,
130 |
| Subjects: | |
| Online Access: | https://doi.org/10.1385/159259686X |
Table of Contents:
- Isolation of Target Gene Promoter/Enhancer Sequences by Whole Genome PCR Method
- In Vivo Footprinting Using UV Light and Ligation-Mediated PCR
- Identification of DNaseI Hypersensitive Sites Within Nuclei
- Analysis of In Vivo Methylation
- Detection of Transcription Factor Partners with a Yeast One Hybrid Screen
- Inverse PCR (IPCR) for Obtaining Promoter Sequence
- PCR-Directed Linker Scanning Mutagenesis
- Transfection Technologies
- The Use of Particle-Mediated Gene Transfer for the Study of Promoter Activity in Somatic Tissues
- Optimizing Electroporation Conditions for the Transformation of Mammalian Cells
- Calcium Phosphate Transfection of Mammalian Cultured Cells
- DEAE-Dextran Transfection of Mammalian Cultured Cells
- Liposome-Mediated Transfection of Mammalian Cells
- Assays for Transcriptional Activity Based on the Luciferase Reporter Gene
- Transient Transfection of Schneider Cells in the Study of Transcription Factors
- Triplex-Forming Oligonucleotides and Their Use in the Analysis of Gene Transcription
- Expression and Purification of Histidine-Tagged Transcription Factors
- Generation of Transcription Factors in Rabbit Reticulocyte Lysate Depleted of Endogenous DNA-Binding Protein
- Electrophoretic Mobility Shift Assays
- In Vitro Promoter Analysis Using Nuclear Extracts and G-Free Cassette Vectors
- In Vitro Transcription Using Competitor Oligonucleotides to Deplete Specific Transcription Factors
- Computer Software of Eukaryotic Promoter Analysis.