mRNA Processing and Metabolism Methods and Protocols /

Detalles Bibliográficos
Autor Corporativo: SpringerLink (Online service)
Otros Autores: Schoenberg, Daniel R. (Editor )
Formato: eBook
Lenguaje:English
Publicado: Totowa, NJ : Humana Press : Imprint: Humana, 2004.
Edición:1st ed. 2004.
Colección:Methods in Molecular Biology, 257
Materias:
Acceso en línea:https://doi.org/10.1385/1592597505
Tabla de Contenidos:
  • Using Chromatin Immunoprecipitation to Map Cotranscriptional mRNA Processing in Saccharomyces cerevisiae
  • Identifying PhosphoCTD-Associating Proteins
  • Imaging Alternative Splicing in Living Cells
  • Tobramycin Affinity Tag Purification of Spliceosomes
  • Using Single-Strand Conformational Polymorphism Gel Electrophoresis to Analyze Mutually Exclusive Alternative Splicing
  • Methods for the Analysis of Adenosine-to-Inosine Editing in RNA
  • Assaying Nuclear Messenger RNA Export in Human Cells
  • RNA Unwinding Assay for DExD/H-Box RNA Helicases
  • Approaches for Monitoring Nuclear Translation
  • Immunopurification and Analysis of Protein and RNA Components of mRNP in Mammalian Cells
  • Gene Expression Analysis of Messenger RNP Complexes
  • Using the ?N Peptide to Tether Proteins to RNAs
  • An Efficient System for Cap- and Poly(A)-Dependent Translation In Vitro
  • A Poly(A) Tail-Responsive In Vitro System for Cap- or IRES-Driven Translation From HeLa Cells
  • Assessing Messenger RNA Decapping in Cellular Extracts
  • Analysis of RNA Exonucleolytic Activities in Cellular Extracts
  • Application of Ligation-Mediated Reverse Transcription Polymerase Chain Reaction to the Identification of In Vivo Endonuclease-Generated Messenger RNA Decay Intermediates
  • Biochemical Dissection of RNA Silencing in Plants
  • RNA Interference of mRNA Processing Factors in Drosophila S2 Cells
  • RNA Interference by Short Hairpin RNAs Expressed in Vertebrate Cells.