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03009nam a22003015i 4500 |
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978-1-59259-754-3 |
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20191026231410.0 |
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cr nn 008mamaa |
| 008 |
100301s2004 xxu| s |||| 0|eng d |
| 020 |
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|a 9781592597543
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| 024 |
7 |
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|a 10.1385/1592597548
|2 doi
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| 040 |
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|a Sistema de Bibliotecas del Tecnológico de Costa Rica
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| 245 |
1 |
0 |
|a Receptor Signal Transduction Protocols
|c edited by Gary B. Willars, R. A. John Challiss.
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| 250 |
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|a 2nd ed. 2004.
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| 260 |
# |
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|a Totowa, NJ :
|b Humana Press :
|b Imprint: Humana,
|c 2004.
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| 300 |
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|a XIII, 418 p.
|b online resource.
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| 336 |
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|a text
|b txt
|2 rdacontent
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| 337 |
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|a computer
|b c
|2 rdamedia
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| 338 |
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|a online resource
|b cr
|2 rdacarrier
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| 490 |
1 |
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|a Methods in Molecular Biology,
|v 259
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| 505 |
0 |
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|a Radioligand-Binding Methods for Membrane Preparations and Intact Cells -- Determination of Allosteric Interactions Using Radioligand-Binding Techniques -- Generation, Use, and Validation of Receptor-Selective Antibodies -- Immunocytochemical Identification of G-Protein-Coupled Receptor Expression and Localization -- Generation and Use of Epitope-Tagged Receptors -- Identification of G-Protein-Coupled Receptor mRNA Expression by Northern Blotting and In Situ Hybridization -- Electron Microscopic Studies of Receptor Localization -- Generation of Model Cell Lines Expressing Recombinant G-Protein-Coupled Receptors -- Viral Infection Protocols -- Expression of G-Protein Signaling Components in Adult Mammalian Neurons by Microinjection -- Covalent Modification of G-Proteins by Affinity Labeling -- Measurement of Agonist-Stimulated [35S]GTP?S Binding to Assess Total G-Protein and G?-Subtype-Specific Activation by G-Protein-Coupled Receptors -- Identification and Quantitation of G-Protein ?-Subunits -- Analysis of Function of Receptor-G-Protein and Receptor-RGS Fusion Proteins -- Assessment of Receptor Internalization and Recycling -- G-Protein-Coupled Receptor Phosphorylation and Palmitoylation -- Identification of G-Protein-Coupled Receptor Phosphorylation Sites by 2D Phosphopeptide Mapping -- Ubiquitination of G-Protein-Coupled Receptors -- Receptor Mutagenesis Strategies for Examination of Structure-Function Relationships -- Study of G-Protein-Coupled Receptor-Protein Interactions by Bioluminescence Resonance Energy Transfer -- Fluorescence Resonance Energy Transfer to Study Receptor Dimerization in Living Cells -- Identification of Protein Interactions by Yeast Two-Hybrid Screening and Coimmunoprecipitation -- Study of G-Protein-Coupled Receptor-Protein Interactions Using Gel Overlay Assays and Glutathione-S-Transferase-Fusion Protein Pull-Downs -- Receptor Knock-Out and Knock-In Strategies -- Statistical Methods in G-Protein-Coupled Receptor Research.
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| 650 |
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|a Cell biology.
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| 650 |
1 |
4 |
|a Cell Biology.
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| 700 |
1 |
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|a Willars, Gary B.
|e editor.
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| 700 |
1 |
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|a Challiss, R. A. John.
|e editor.
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| 710 |
2 |
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|a SpringerLink (Online service)
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| 773 |
0 |
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|t Springer eBooks
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| 856 |
4 |
0 |
|u https://doi.org/10.1385/1592597548
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