Protein'Protein Interactions Methods and Applications /

Protein'Protein Interactions Methods and Applications /

Detalles Bibliográficos
Autor Corporativo: SpringerLink (Online service)
Otros Autores: Fu, Haian. (Editor )
Formato: eBook
Lenguaje:English
Publicado: Totowa, NJ : Humana Press : Imprint: Humana, 2004.
Edición:1st ed. 2004.
Colección:Methods in Molecular Biology, 261
Materias:
Biochemistry.
Biochemistry, general.
Acceso en línea:https://doi.org/10.1385/1592597629
Tabla de Contenidos:
  • Overview
  • Structural Basis of Protein-Protein Interactions
  • Quantitative Analysis of Protein-Protein Interactions
  • In Vitro Techniques
  • Characterization of Protein-Protein Interactions by Isothermal Titration Calorimetry
  • Circular Dichroism Analysis for Protein-Protein Interactions
  • Protein-Protein Interaction Analysis by Nuclear Magnetic Resonance Spectroscopy
  • Measuring Rhodopsin-G-Protein Interactions by Surface Plasmon Resonance
  • Using Light Scattering to Determine the Stoichiometry of Protein Complexes
  • Sedimentation Equilibrium Studies
  • Analysis of Protein-Protein Interactions by Simulation of Small-Zone Gel Filtration Chromatography
  • Fluorescence Gel Retardation Assay to Detect Protein-Protein Interactions
  • Fluorescence Polarization Assay to Quantify Protein-Protein Interactions
  • Studying Protein-Protein Interactions via Blot Overlay or Far Western Blot
  • Glutathione-S-Transferase-Fusion Based Assays for Studying Protein-Protein Interactions
  • Affinity Capillary Electrophoresis Analyses of Protein-Protein Interactions in Target-Directed Drug Discovery
  • Mapping Protein-Ligand Interactions by Hydroxyl-Radical Protein Footprinting
  • Use of Phage Display and Polyvalency to Design Inhibitors of Protein-Protein Interactions
  • Detecting Protein-Protein Interactions in Heterologous Systems
  • A Bacterial Two-Hybrid System Based on Transcription Activation
  • Using the Yeast Two-Hybrid System to Identify Interacting Proteins
  • Analysis of Protein-Protein Interactions Utilizing Dual Bait Yeast Two-Hybrid System
  • The Split-Ubiquitin Membrane-Based Yeast Two-Hybrid System
  • Reverse Two-Hybrid Techniques in the Yeast Saccharomyces cerevisiae
  • Mammalian Two-Hybrid Assay for Detecting Protein-Protein Interactions In Vivo
  • Co-Immunoprecipitation from Transfected Cells
  • Probing Protein-Protein Interactions in Living Cells
  • Microscopic Analysis of Fluorescence Resonance Energy Transfer (FRET)
  • Monitoring Molecular Interactions in Living Cells Using Flow Cytometric Analysis of Fluorescence Resonance Energy Transfer
  • Fluorescence Correlation Spectroscopy
  • Confocal Microscopy for Intracellular Co-Localization of Proteins
  • Mapping Biochemical Networks With Protein-Fragment Complementation Assays
  • In Vivo Protein Cross-Linking
  • Proteomics-Based Approaches
  • Computational Prediction of Protein-Protein Interactions
  • Affinity Methods for Phosphorylation-Dependent Interactions
  • Two-Dimensional Gel Electrophoresis for Analysis of Protein Complexes
  • Sample Preparation of Gel Electrophoretically Separated Protein Binding Partners for Analysis by Mass Spectrometry
  • Quantitative Protein Analysis by Solid Phase Isotope Tagging and Mass Spectrometry
  • Internet Resources for Studying Protein-Protein Interactions.

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