Recombinant Gene Expression Reviews and Protocols /

Detalles Bibliográficos
Autor Corporativo: SpringerLink (Online service)
Otros Autores: Balbas, Paulina. (Editor ), Lorence, Argelia. (Editor )
Formato: eBook
Lenguaje:English
Publicado: Totowa, NJ : Humana Press : Imprint: Humana, 2004.
Edición:2nd ed. 2004.
Colección:Methods in Molecular Biology, 267
Materias:
Acceso en línea:https://doi.org/10.1385/1592597742
Tabla de Contenidos:
  • General Issues About Recombinant Gene Expression
  • Host Cell Compatibility in Protein Expression
  • Production of Recombinant Proteins
  • Folding-Promoting Agents in Recombinant Protein Production
  • Prokaryotes
  • Back to Basics
  • ?-Complementation-Enabled T7 Expression Vectors and Their Use for the Expression of Recombinant Polypeptides for Protein Transduction Experiments
  • Expression of Recombinant Alkaline Phosphatase Conjugates in Escherichia coli
  • Overexpression of Chromosomal Genes in Escherichia coli
  • Chromosomal Expression of Foreign and Native Genes From Regulatable Promoters in Escherichia coli
  • Plasmid Vectors for Marker-Free Chromosomal Insertion of Genetic Material in Escherichia coli
  • Copy-Control pBAC/oriV Vectors for Genomic Cloning
  • Copy-Control Tightly Regulated Expression Vectors Based on pBAC/oriV
  • Cell-Free Protein Synthesis With Prokaryotic Combined Transcription-Translation
  • Genetic Tools for the Manipulation of Moderately Halophilic Bacteria of the Family Halomonadaceae
  • Gene Transfer and Expression of Recombinant Proteins in Moderately Halophilic Bacteria
  • Recombinant Protein Production in Antarctic Gram-Negative Bacteria
  • Fungi
  • Recombinant Protein Production in Yeasts
  • Controlled Expression of Homologous Genes by Genomic Promoter Replacement in the Yeast Saccharomyces cerevisiae
  • High-Throughput Expression in Microplate Format in Saccharomyces cerevisiae
  • High-Throughput Expression in Microplate Format in Pichia pastoris
  • Multiple Gene Expression by Chromosomal Integration and CRE-loxP-Mediated Marker Recycling in Saccharomyces cerevisiae
  • Three Decades of Fungal Transformation
  • Three Decades of Fungal Transformation
  • Plants and Plant Cells
  • Gene Transfer and Expression in Plants
  • Production of Recombinant Proteins by Hairy Roots Cultured in Plastic Sleeve Bioreactors
  • Engineering the Chloroplast Genome for Hyperexpression of Human Therapeutic Proteins and Vaccine Antigens
  • New Selection Marker for Plant Transformation
  • Enhancer Detection and Gene Trapping as Tools for Functional Genomics in Plants
  • Animals and Animal Cells
  • Gene Transfer and Expression in Mammalian Cell Lines and Transgenic Animals
  • Sustained Heterologous Transgene Expression in Mammalian and Avian Cell Lines
  • Inducible Gene Expression in Mammalian Cells and Mice
  • Flp-Mediated Integration of Expression Cassettes into FRT-Tagged Chromosomal Loci in Mammalian Cells
  • Generation of High-Recombinant-Protein-Producing Chinese Hamster Ovary (CHO) Cells
  • Preparation of Recombinant Proteins in Milk.