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02937nam a22003015i 4500 |
| 001 |
978-1-59259-783-3 |
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20191027042253.0 |
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cr nn 008mamaa |
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100301s2004 xxu| s |||| 0|eng d |
| 020 |
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|a 9781592597833
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| 024 |
7 |
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|a 10.1385/1592597831
|2 doi
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| 040 |
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|a Sistema de Bibliotecas del Tecnológico de Costa Rica
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| 245 |
1 |
0 |
|a Platelets and Megakaryocytes
|b Volume 2: Perspectives and Techniques /
|c edited by Jonathan M. Gibbins, Martyn P. Mahaut-Smith.
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| 250 |
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|a 1st ed. 2004.
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| 260 |
# |
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|a Totowa, NJ :
|b Humana Press :
|b Imprint: Humana,
|c 2004.
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| 300 |
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|a XX, 504 p. 113 illus., 5 illus. in color.
|b online resource.
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| 336 |
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|a text
|b txt
|2 rdacontent
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| 337 |
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|a computer
|b c
|2 rdamedia
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| 338 |
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|a online resource
|b cr
|2 rdacarrier
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| 490 |
1 |
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|a Methods in Molecular Biology,
|v 273
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| 505 |
0 |
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|a Perspectives -- Signaling Receptors on Platelets and Megakaryocytes -- Manipulation of Gene Expression in Megakaryocytes -- In Vitro Changes of Platelet Parameters -- Pharmacological Approaches to Studying Platelet Function -- Inhibitors of Cellular Signaling Targets -- Signaling Techniques -- Ligand-Binding Assays for Collagen -- Use of Radiolabeled 2-Methylthio-ADP to Study P2Y Receptors on Platelets and Cell Lines -- Ligand-Binding Assays -- Techniques for Analysis of Proteins by SDS-Polyacrylamide Gel Electrophoresis and Western Blotting -- Study of Tyrosine Kinases and Protein Tyrosine Phosphorylation -- Protein Tyrosine Phosphatases -- The Study of Serine-Threonine Kinases -- Phosphoinositides -- Isolation and Analysis of Platelet Lipid Rafts -- Measurement and Manipulation of [Ca2+]i in Suspensions of Platelets and Cell Cultures -- Measurement and Manipulation of Intracellular Ca2+ in Single Platelets and Megakaryocytes -- Patch-Clamp Recordings of Electrophysiological Events in the Platelet and Megakaryocyte -- Evaluation of Nitrotyrosine-Containing Proteins in Blood Platelets -- Nitric Oxide Signaling in Platelets -- Snake Venom Toxins Affecting Platelet Function -- Peptide Synthesis in the Study of Collagen-Platelet Interactions -- Platelet Permeabilization -- Molecular and Post-Genomic Techniques -- Using Retroviruses to Express Genes in Primary Megakaryocyte Lineage Cells -- Use of Antisense Oligonucleotide Technology to Investigate Signaling Pathways in Megakaryocytes -- GFP Fusion Proteins to Study Signaling in Live Cells -- Two-Dimensional Polyacrylamide Gel Electrophoresis for Platelet Proteomics -- Preparation of mRNA and cDNA Libraries From Platelets and Megakaryocytes -- Platelet Receptor Structures and Polymorphisms -- Gene Array Technology and the Study of Platelets and Megakaryocytes.
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| 650 |
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|a Cell biology.
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| 650 |
1 |
4 |
|a Cell Biology.
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| 700 |
1 |
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|a Gibbins, Jonathan M.
|e editor.
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| 700 |
1 |
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|a Mahaut-Smith, Martyn P.
|e editor.
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| 710 |
2 |
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|a SpringerLink (Online service)
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| 773 |
0 |
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|t Springer eBooks
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| 856 |
4 |
0 |
|u https://doi.org/10.1385/1592597831
|