Platelets and Megakaryocytes Volume 2: Perspectives and Techniques /

Detalles Bibliográficos
Autor Corporativo: SpringerLink (Online service)
Otros Autores: Gibbins, Jonathan M. (Editor ), Mahaut-Smith, Martyn P. (Editor )
Formato: eBook
Lenguaje:English
Publicado: Totowa, NJ : Humana Press : Imprint: Humana, 2004.
Edición:1st ed. 2004.
Colección:Methods in Molecular Biology, 273
Materias:
Acceso en línea:https://doi.org/10.1385/1592597831
Tabla de Contenidos:
  • Perspectives
  • Signaling Receptors on Platelets and Megakaryocytes
  • Manipulation of Gene Expression in Megakaryocytes
  • In Vitro Changes of Platelet Parameters
  • Pharmacological Approaches to Studying Platelet Function
  • Inhibitors of Cellular Signaling Targets
  • Signaling Techniques
  • Ligand-Binding Assays for Collagen
  • Use of Radiolabeled 2-Methylthio-ADP to Study P2Y Receptors on Platelets and Cell Lines
  • Ligand-Binding Assays
  • Techniques for Analysis of Proteins by SDS-Polyacrylamide Gel Electrophoresis and Western Blotting
  • Study of Tyrosine Kinases and Protein Tyrosine Phosphorylation
  • Protein Tyrosine Phosphatases
  • The Study of Serine-Threonine Kinases
  • Phosphoinositides
  • Isolation and Analysis of Platelet Lipid Rafts
  • Measurement and Manipulation of [Ca2+]i in Suspensions of Platelets and Cell Cultures
  • Measurement and Manipulation of Intracellular Ca2+ in Single Platelets and Megakaryocytes
  • Patch-Clamp Recordings of Electrophysiological Events in the Platelet and Megakaryocyte
  • Evaluation of Nitrotyrosine-Containing Proteins in Blood Platelets
  • Nitric Oxide Signaling in Platelets
  • Snake Venom Toxins Affecting Platelet Function
  • Peptide Synthesis in the Study of Collagen-Platelet Interactions
  • Platelet Permeabilization
  • Molecular and Post-Genomic Techniques
  • Using Retroviruses to Express Genes in Primary Megakaryocyte Lineage Cells
  • Use of Antisense Oligonucleotide Technology to Investigate Signaling Pathways in Megakaryocytes
  • GFP Fusion Proteins to Study Signaling in Live Cells
  • Two-Dimensional Polyacrylamide Gel Electrophoresis for Platelet Proteomics
  • Preparation of mRNA and cDNA Libraries From Platelets and Megakaryocytes
  • Platelet Receptor Structures and Polymorphisms
  • Gene Array Technology and the Study of Platelets and Megakaryocytes.