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02501nam a22002895i 4500 |
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978-1-59259-793-2 |
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20190618131522.0 |
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cr nn 008mamaa |
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100301s2004 xxu| s |||| 0|eng d |
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|a 9781592597932
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|a 10.1385/1592597939
|2 doi
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|a Sistema de Bibliotecas del Tecnológico de Costa Rica
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|a Parasite Genomics Protocols
|c edited by Sara E. Melville.
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| 250 |
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|a 1st ed. 2004.
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| 260 |
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|a Totowa, NJ :
|b Humana Press :
|b Imprint: Humana,
|c 2004.
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| 300 |
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|a XVI, 452 p.
|b online resource.
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| 336 |
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|a text
|b txt
|2 rdacontent
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| 337 |
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|a computer
|b c
|2 rdamedia
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| 338 |
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|a online resource
|b cr
|2 rdacarrier
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| 490 |
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|a Methods in Molecular Biology,
|v 270
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|a Sequencing Strategies for Parasite Genomes -- Annotation of Parasite Genomes -- Parasite Genome Databases and Web-Based Resources -- Expressed Sequence Tags -- Expressed Sequence Tags -- Positive Selection Scanning of Parasite DNA Sequences -- RACE and RAGE Cloning in Parasitic Microbial Eukaryotes -- Amplified (Restriction) Fragment Length Polymorphism (AFLP) Analysis -- Minisatellites and MVR-PCR for the Individual Identification of Parasite Isolates -- Analysis of Differentially Expressed Parasite Genes and Proteins Using Transcriptomics and Proteomics -- Gene Expression Studies Using Self-Fabricated Parasite cDNA Microarrays -- DNA Content Analysis on Microarrays -- Typing Single-Nucleotide Polymorphisms in Toxoplasma gondii by Allele-Specific Primer Extension and Microarray Detection -- Transfection of the Human Malaria Parasite Plasmodium falciparum -- A PCR-Based Method for Gene Deletion and Protein Tagging in Trypanosoma brucei -- Analysis of Gene Function in Trypanosoma brucei Using RNA Interference -- In Vitro Shuttle Mutagenesis Using Engineered Mariner Transposons -- Random Mutagenesis Strategies for Construction of Large and Diverse Clone Libraries of Mutated DNA Fragments -- Separation, Digestion, and Cloning of Intact Parasite Chromosomes Embedded in Agarose -- Chromosome Fragmentation in Leishmania -- FISH Mapping for Helminth Genome -- Fiber-FISH: Fluorescence In Situ Hybridization on Stretched DNA -- Yeast Two-Hybrid Assay for Studying Protein-Protein Interactions -- From Genomes to Vaccines for Leishmaniasis.
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| 650 |
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|a Microbiology.
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| 650 |
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4 |
|a Microbiology.
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| 700 |
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|a Melville, Sara E.
|e editor.
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| 710 |
2 |
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|a SpringerLink (Online service)
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| 773 |
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|t Springer eBooks
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| 856 |
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|u https://doi.org/10.1385/1592597939
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