Ubiquitin-Proteasome Protocols
| Corporate Author: | |
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| Other Authors: | , |
| Format: | eBook |
| Language: | English |
| Published: |
Totowa, NJ :
Humana Press : Imprint: Humana,
2005.
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| Edition: | 1st ed. 2005. |
| Series: | Methods in Molecular Biology,
301 |
| Subjects: | |
| Online Access: | https://doi.org/10.1385/1592598951 |
Table of Contents:
- Biochemical Methods to Study the Ubiquitin-Proteasome System
- Small-Molecule Inhibitors of Proteasome Activity
- Purification of E1 and E1-Like Enzymes
- Assays for RING Family Ubiquitin Ligases
- Ubiquitin Chain Synthesis
- Purification of Proteasomes, Proteasome Subcomplexes, and Proteasome-Associated Proteins From Budding Yeast
- Recognition and Processing of Misfolded Proteins by PA700, the 19S Regulatory Complex of the 26S Proteasome
- Cell-Free Assay for Ubiquitin-Independent Proteasomal Protein Degradation
- Assays of Proteasome-Dependent Cleavage Products
- Identification of Components of Protein Complexes
- Mass Spectrometric Determination of Protein Ubiquitination
- Reconstitution of Endoplasmic Reticulum-Associated Degradation Using Yeast Membranes and Cytosol
- Reticulocyte Lysate as a Model System to Study Endoplasmic Reticulum Membrane Protein Degradation
- Deubiquitinating Enzyme Purification, Assay Inhibitors, and Characterization
- Cellular Methods to Study Ubiquitin-Proteasome-Dependent Functions
- Measuring Ubiquitin Conjugation in Cells
- Assays for Proteasome Assembly and Maturation
- N-Terminal Ubiquitination
- Quantitating Defective Ribosome Products
- Endoplasmic Reticulum-Associated Protein Quality Control and Degradation
- Endoplasmic Reticulum-Associated Protein Quality Control and Degradation
- Cystic Fibrosis Transmembrane Conductance Regulator as a Model Substrate to Study Endoplasmic Reticulum Protein Quality Control in Mammalian Cells
- Aggresome Formation
- Detection of Sumoylated Proteins
- Proteasome Inhibitors in Cancer Therapy
- Parkinson's Disease.