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03888nam a22003015i 4500 |
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978-1-59259-922-6 |
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20190619050209.0 |
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cr nn 008mamaa |
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100301s2005 xxu| s |||| 0|eng d |
| 020 |
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|a 9781592599226
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| 024 |
7 |
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|a 10.1385/1592599222
|2 doi
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| 040 |
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|a Sistema de Bibliotecas del Tecnológico de Costa Rica
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| 245 |
1 |
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|a Therapeutic Proteins
|b Methods and Protocols /
|c edited by C. Mark Smales, David C. James.
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| 250 |
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|a 1st ed. 2005.
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| 260 |
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|a Totowa, NJ :
|b Humana Press :
|b Imprint: Humana,
|c 2005.
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| 300 |
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|a XIV, 482 p.
|b online resource.
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| 336 |
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|a text
|b txt
|2 rdacontent
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| 337 |
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|a computer
|b c
|2 rdamedia
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| 338 |
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|a online resource
|b cr
|2 rdacarrier
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| 490 |
1 |
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|a Methods in Molecular Biology,
|v 308
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| 505 |
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|a Biopharmaceutical Proteins -- Expression of Antibody Fragments by Periplasmic Secretion in Escherichia coli -- Secretory Production of Therapeutic Proteins in Escherichia coli -- Expression of Recombinant LTB Protein in Marine vibrio VSP60 -- Heterologous Gene Expression in Yeast -- Pharmaceutical Proteins From Methylotrophic Yeasts -- Expression of Human Papillomavirus Type 16 L1 in Baculovirus Expression Systems -- Large-Scale Transient Expression of Therapeutic Proteins in Mammalian Cells -- Site-Specific Integration for High-Level Protein Production in Mammalian Cells -- Production of Recombinant Therapeutic Proteins by Mammalian Cells in Suspension Culture -- Transgene Control Engineering in Mammalian Cells -- Fusion to Albumin as a Means to Slow the Clearance of Small Therapeutic Proteins Using the Pichia pastoris Expression System -- High-Throughput Recovery of Therapeutic Proteins from the Inclusion Bodies of Escherichia coli -- Isolation, Solubilization, Refolding, and Chromatographic Purification of Human Growth Hormone from Inclusion Bodies of Escherichia coli Cells -- Large-Scale Preparation of Factor VIIa from Human Plasma -- Purification of Clinical-Grade Monoclonal Antibodies by Chromatographic Methods -- Virus Elimination and Validation -- Virus Removal by Nanofiltration -- Determining Residual Host Cell Antigen Levels in Purified Recombinant Proteins by Slot Blot and Scanning Laser Densitometry -- Principles of Biopharmaceutical Protein Formulation -- Solid-State Protein Formulation -- Stabilization of Therapeutic Proteins by Chemical and Physical Methods -- Extraction and Characterization of Vaccine Antigens from Water-in-Oil Adjuvant Formulations -- Probing Reversible Self-Association of Therapeutic Proteins by Sedimentation Velocity in the Analytical Ultracentrifuge -- Biological Characterization of Pegylated Interferons -- Characterization of Interferon ?2B Pegylated via Carboxyalkylation -- Quantifying Recombinant Proteins and Their Degradation Products Using SDS-PAGE and Scanning Laser Densitometry -- Extraction and Denaturing Gel Electrophoretic Methodology for the Analysis of Yeast Proteins -- Characterization of Therapeutic Proteins by Membrane and In-Gel Tryptic Digestion -- Oligosaccharide Release and MALDI-TOF MS Analysis of N-Linked Carbohydrate Structures from Glycoproteins -- Capillary Electrophoresis of Carbohydrates Derivatized With Fluorophoric Compounds -- High-Throughput LC/MS Methodology for ?(1?3)Gal Determination of Recombinant Monoclonal Antibodies -- Carbohydrate Structural Characterization of Fas Ligand Inhibitory Protein -- Top-Down Characterization of Protein Pharmaceuticals by Liquid Chromatography/Mass Spectrometry -- Sample Preparation Procedures for High-Resolution Nuclear Magnetic Resonance Studies of Aqueous and Stabilized Solutions of Therapeutic Peptides.
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| 650 |
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|a Biochemistry.
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| 650 |
1 |
4 |
|a Biochemistry, general.
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| 700 |
1 |
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|a Smales, C. Mark.
|e editor.
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| 700 |
1 |
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|a James, David C.
|e editor.
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| 710 |
2 |
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|a SpringerLink (Online service)
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| 773 |
0 |
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|t Springer eBooks
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| 856 |
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|u https://doi.org/10.1385/1592599222
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