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02577nam a22003135i 4500 |
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978-1-59745-005-8 |
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20191027151119.0 |
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cr nn 008mamaa |
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100301s2006 xxu| s |||| 0|eng d |
| 020 |
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|a 9781597450058
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| 024 |
7 |
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|a 10.1385/1597450057
|2 doi
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| 040 |
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|a Sistema de Bibliotecas del Tecnológico de Costa Rica
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| 245 |
1 |
0 |
|a Nuclear Reprogramming
|b Methods and Protocols /
|c edited by Steve Pells.
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| 250 |
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|a 1st ed. 2006.
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| 260 |
# |
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|a Totowa, NJ :
|b Humana Press :
|b Imprint: Humana,
|c 2006.
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| 300 |
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|a XIV, 334 p.
|b online resource.
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| 336 |
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|a text
|b txt
|2 rdacontent
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| 337 |
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|a computer
|b c
|2 rdamedia
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| 338 |
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|a online resource
|b cr
|2 rdacarrier
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| 490 |
1 |
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|a Methods in Molecular Biology,
|v 325
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| 505 |
0 |
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|a Nuclear Transplantation in Xenopus -- Nuclear Transfer in Sheep -- Protocols for Nuclear Transfer in Mice -- Isolation of Stromal Stem Cells From Human Adipose Tissue -- Nuclear Reprogramming by Cell Fusion -- Polyethylene Glycol-Mediated Cell Fusion -- Epigenetic Reprogramming of Somatic Genomes by Electrofusion With Embryonic Stem Cells -- Quantification of Cell Fusion by Flow Cytometry -- Modulation of Cell Fate Using Nuclear and Cytoplasmic Extracts -- Transgenic Systems in Nuclear Reprogramming -- Using Immunofluorescence to Observe Methylation Changes in Mammalian Preimplantation Embryos -- Observing S-Phase Dynamics of Histone Modifications With Fluorescently Labeled Antibodies -- Quantitative Analysis of Telomerase Activity and Telomere Length in Domestic Animal Clones -- Pluripotency: Capacity for In Vitro Differentiation of Undifferentiated Embryonic Stem Cells -- Staining Embryonic Stem Cells Using Monoclonal Antibodies to Stage-Specific Embryonic Antigens -- Analysis of the Nucleolar Compartment of the Nucleus as an Indicator of Nuclear Reprogramming After Nuclear Transfer -- Methylation-Sensitive Polymerase Chain Reaction -- Analysis of DNA Methylation Profiles in Preimplantation Embryos Using Bisulfite Mutagenesis -- Chromatin Immunoprecipitation Assay for Mammalian Tissues -- Histone Modifications and Transcription Factor Binding on Chromatin: ChIP–PCR Assays -- In Vivo Genomic Footprinting Using LM–PCR Methods -- Analyzing Histone Modification Using Crosslinked Chromatin Treated With Micrococcal Nuclease.
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| 650 |
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0 |
|a Biochemistry.
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| 650 |
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0 |
|a Cell biology.
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| 650 |
1 |
4 |
|a Biochemistry, general.
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| 650 |
2 |
4 |
|a Cell Biology.
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| 700 |
1 |
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|a Pells, Steve.
|e editor.
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| 710 |
2 |
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|a SpringerLink (Online service)
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| 773 |
0 |
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|t Springer eBooks
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| 856 |
4 |
0 |
|u https://doi.org/10.1385/1597450057
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