Nuclear Reprogramming Methods and Protocols /

Detalles Bibliográficos
Autor Corporativo: SpringerLink (Online service)
Otros Autores: Pells, Steve. (Editor )
Formato: eBook
Lenguaje:English
Publicado: Totowa, NJ : Humana Press : Imprint: Humana, 2006.
Edición:1st ed. 2006.
Colección:Methods in Molecular Biology, 325
Materias:
Acceso en línea:https://doi.org/10.1385/1597450057
Tabla de Contenidos:
  • Nuclear Transplantation in Xenopus
  • Nuclear Transfer in Sheep
  • Protocols for Nuclear Transfer in Mice
  • Isolation of Stromal Stem Cells From Human Adipose Tissue
  • Nuclear Reprogramming by Cell Fusion
  • Polyethylene Glycol-Mediated Cell Fusion
  • Epigenetic Reprogramming of Somatic Genomes by Electrofusion With Embryonic Stem Cells
  • Quantification of Cell Fusion by Flow Cytometry
  • Modulation of Cell Fate Using Nuclear and Cytoplasmic Extracts
  • Transgenic Systems in Nuclear Reprogramming
  • Using Immunofluorescence to Observe Methylation Changes in Mammalian Preimplantation Embryos
  • Observing S-Phase Dynamics of Histone Modifications With Fluorescently Labeled Antibodies
  • Quantitative Analysis of Telomerase Activity and Telomere Length in Domestic Animal Clones
  • Pluripotency: Capacity for In Vitro Differentiation of Undifferentiated Embryonic Stem Cells
  • Staining Embryonic Stem Cells Using Monoclonal Antibodies to Stage-Specific Embryonic Antigens
  • Analysis of the Nucleolar Compartment of the Nucleus as an Indicator of Nuclear Reprogramming After Nuclear Transfer
  • Methylation-Sensitive Polymerase Chain Reaction
  • Analysis of DNA Methylation Profiles in Preimplantation Embryos Using Bisulfite Mutagenesis
  • Chromatin Immunoprecipitation Assay for Mammalian Tissues
  • Histone Modifications and Transcription Factor Binding on Chromatin: ChIP–PCR Assays
  • In Vivo Genomic Footprinting Using LM–PCR Methods
  • Analyzing Histone Modification Using Crosslinked Chromatin Treated With Micrococcal Nuclease.