Nuclear Reprogramming Methods and Protocols /
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| Format: | eBook |
| Language: | English |
| Published: |
Totowa, NJ :
Humana Press : Imprint: Humana,
2006.
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| Edition: | 1st ed. 2006. |
| Series: | Methods in Molecular Biology,
325 |
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| Online Access: | https://doi.org/10.1385/1597450057 |
Table of Contents:
- Nuclear Transplantation in Xenopus
- Nuclear Transfer in Sheep
- Protocols for Nuclear Transfer in Mice
- Isolation of Stromal Stem Cells From Human Adipose Tissue
- Nuclear Reprogramming by Cell Fusion
- Polyethylene Glycol-Mediated Cell Fusion
- Epigenetic Reprogramming of Somatic Genomes by Electrofusion With Embryonic Stem Cells
- Quantification of Cell Fusion by Flow Cytometry
- Modulation of Cell Fate Using Nuclear and Cytoplasmic Extracts
- Transgenic Systems in Nuclear Reprogramming
- Using Immunofluorescence to Observe Methylation Changes in Mammalian Preimplantation Embryos
- Observing S-Phase Dynamics of Histone Modifications With Fluorescently Labeled Antibodies
- Quantitative Analysis of Telomerase Activity and Telomere Length in Domestic Animal Clones
- Pluripotency: Capacity for In Vitro Differentiation of Undifferentiated Embryonic Stem Cells
- Staining Embryonic Stem Cells Using Monoclonal Antibodies to Stage-Specific Embryonic Antigens
- Analysis of the Nucleolar Compartment of the Nucleus as an Indicator of Nuclear Reprogramming After Nuclear Transfer
- Methylation-Sensitive Polymerase Chain Reaction
- Analysis of DNA Methylation Profiles in Preimplantation Embryos Using Bisulfite Mutagenesis
- Chromatin Immunoprecipitation Assay for Mammalian Tissues
- Histone Modifications and Transcription Factor Binding on Chromatin: ChIP-PCR Assays
- In Vivo Genomic Footprinting Using LM-PCR Methods
- Analyzing Histone Modification Using Crosslinked Chromatin Treated With Micrococcal Nuclease.