Plant Virology Protocols From Viral Sequence to Protein Function /
| Corporate Author: | |
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| Other Authors: | , , , |
| Format: | eBook |
| Language: | English |
| Published: |
Totowa, NJ :
Humana Press : Imprint: Humana,
2008.
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| Edition: | 2nd ed. 2008. |
| Series: | Methods in Molecular Biology,
451 |
| Subjects: | |
| Online Access: | https://doi.org/10.1007/978-1-59745-102-4 |
Table of Contents:
- General Introduction
- Plant-Virus Interactions
- Role of Capsid Proteins
- Role of Plant Virus Movement Proteins
- Multiple Roles of Viral Replication Proteins in Plant RNA Virus Replication
- Role of Silencing Suppressor Proteins
- Role of Vector-Transmission Proteins
- Viral Nucleic Acid Sequence/Function Analysis
- In Vivo Analyses of Viral RNA Translation
- In Vitro Analysis of Translation Enhancers
- Identification of Plant Virus IRES
- Analysis of Geminivirus DNA Replication by 2-D Gel
- Begomoviruses: Molecular Cloning and Identification of Replication Origin
- Analysis of Viroid Replication
- Biochemical Analyses of the Interactions Between Viral Polymerases and RNAs
- In Situ Detection of Plant Viruses and Virus-Specific Products
- Detection of siRNAs and miRNAs
- Cloning of Short Interfering RNAs from Virus-Infected Plants
- Solution Structure Probing of RNA Structures
- RNA Encapsidation Assay
- Protein Analysis and Investigation of Protein Function
- Surface Plasmon Resonance Analysis of Interactions Between Replicase Proteins of Tomato Bushy Stunt Virus
- Biochemical Approaches for Characterizing RNA-Protein Complexes in Preparation for High Resolution Structure Analysis
- Probing Interactions Between Plant Virus Movement Proteins and Nucleic Acids
- Movement Profiles: A Tool for Quantitative Analysis of Cell-to-Cell Movement of Plant Viral Movement Proteins
- Analysis of siRNA-Suppressor of Gene Silencing Interactions
- Phosphorylation Analysis of Plant Viral Proteins
- Analysis of Interactions Between Viral Replicase Proteins and Plant Intracellular Membranes
- Membrane and Protein Dynamics in Virus-Infected Plant Cells
- Site-Directed Mutagenesis of Whole Viral Genomes
- Viral Protein-Nucleic Acid Interaction: South (North)-Western Blot
- Protein-Protein Interactions: The Yeast Two-Hybrid System
- NMR Analysis of Viral Protein Structures
- Localization of Viral Proteins in Plant Cells: Protein Tagging
- Microscopy/GFP/Protein Tagging/Infections Clones and Other Such Tools
- Construction of Infectious Clones for RNA Viruses: TMV
- Construction of Infectious cDNA Clones for RNA Viruses: Turnip Crinkle Virus
- Construction of Infectious Clones for DNA Viruses: Mastreviruses
- Construction of Infectious Clones of Double-Stranded DNA Viruses of Plants Using Citrus Yellow Mosaic Virus as an Example
- Insertion of Introns: A Strategy to Facilitate Assembly of Infectious Full Length Clones
- Analysis of Cell-to-Cell and Long-Distance Movement of Apple Latent Spherical Virus in Infected Plants Using Green, Cyan, and Yellow Fluorescent Proteins
- Agroinoculation: A Simple Procedure for Systemic Infection of Plants with Viruses
- Geminivirus: Biolistic Inoculation and Molecular Diagnosis
- Genomics, Host Factors and Plant Based Studies
- Expression Microarrays in Plant-Virus Interaction
- Genome-Wide Screens for Identification of Host Factors in Viral Replication
- Phosphorylation of Movement Proteins by the Plasmodesmal-Associated Protein Kinase
- Virus-Induced Gene Silencing as a Tool to Identify Host Genes Affecting Viral Pathogenicity
- Yeast Two-Hybrid Assay to Identify Host-Virus Interactions.