Osteoporosis Methods and Protocols /
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| Other Authors: | |
| Format: | eBook |
| Language: | English |
| Published: |
Totowa, NJ :
Humana Press : Imprint: Humana,
2008.
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| Edition: | 1st ed. 2008. |
| Series: | Methods in Molecular Biology,
455 |
| Subjects: | |
| Online Access: | https://doi.org/10.1007/978-1-59745-104-8 |
Table of Contents:
- In Vitro Models
- Isolation and Culture of Rodent Osteoprogenitor Cells
- Osteoclast Culture and Resorption Assays
- Assessing New Bone Formation in Neonatal Calvarial Organ Cultures
- Detection of Apoptosis of Bone Cells In Vitro
- In Vivo Rodent Models
- In Vivo Parathyroid Hormone Treatments and RNA Isolation and Analysis
- Assessment of Bone Formation Capacity Using In vivo Transplantation Assays: Procedure and Tissue Analysis
- Ossicle and Vossicle Implant Model Systems
- Utility of the Ovariectomized Rat as a Model for Human Osteoporosis in Drug Discovery
- Orchidectomy Models of Osteoporosis
- Methods of Gene Delivery
- Gene Delivery by Adenoviruses
- Lentivirus Delivery of shRNA Constructs into Osteoblasts
- Gene Delivery by Retroviruses
- Molecular and Genetic Techniques
- Chromatin Immunoprecipitation Assays: Application of ChIP-on-Chip for Defining Dynamic Transcriptional Mechanisms in Bone Cells
- Identification of Transcription Factor Target Genes by ChIP Display
- Application of the Laser Capture Microdissection Technique for Molecular Definition of Skeletal Cell Differentiation In Vivo
- Quantitative Trait Loci Mapping
- Imaging Techniques
- In Situ Nuclear Organization of Regulatory Machinery
- Bioluminescent Imaging in Bone
- Micro-Computed Tomography: A Method for the Non-Destructive Evaluation of the Three-Dimensional Structure of Biological Specimens
- Fourier Transform-Infrared Microspectroscopy and Microscopic Imaging
- Mechanical Testing
- Assessment of the In Vivo Adaptive Response to Mechanical Loading
- Determination of Bone's Mechanical Matrix Properties by Nanoindentation
- Fluid Flow Assays.