MicroRNA Protocols

Detalles Bibliográficos
Autor Corporativo: SpringerLink (Online service)
Otros Autores: Ying, Shao-Yao. (Editor )
Formato: eBook
Lenguaje:English
Publicado: Totowa, NJ : Humana Press : Imprint: Humana, 2006.
Edición:1st ed. 2006.
Colección:Methods in Molecular Biology, 342
Materias:
Acceso en línea:https://doi.org/10.1385/1597451231
Tabla de Contenidos:
  • The MicroRNA: Overview of the RNA Gene That Modulates Gene Functions
  • Structure Analysis of MicroRNA Precursors
  • MicroRNA Biogenesis: Isolation and Characterization of the Microprocessor Complex
  • Recognition and Cleavage of Primary MicroRNA Transcripts
  • Mouse Embryonic Stem Cells as a Model Genetic System to Dissect and Exploit the RNA Interference Machinery
  • MicroRNAs and Messenger RNA Turnover
  • Prediction of MicroRNA Targets
  • Prediction of Human MicroRNA Targets
  • Complications in Mammalian MicroRNA Target Prediction
  • miRBase:The MicroRNA Sequence Database
  • Methodologies for High-Throughput Expression Profiling of MicroRNAs
  • In Situ Hybridization as a Tool to Study the Role of MicroRNAs in Plant Development
  • Usefulness of the Luciferase Reporter System to Test the Efficacy of siRNA
  • Cloning MicroRNAs From Mammalian Tissues
  • Methods for Analyzing MicroRNA Expression and Function During Hematopoietic Lineage Differentiation
  • Identifying MicroRNA Regulators of Cell Death in Drosophila
  • MicroRNAs in Human Immunodeficiency Virus-1 Infection
  • Cloning and Detection of HIV-1-Encoded MicroRNA
  • Identification of Messenger RNAs and MicroRNAs Associated With Fragile X Mental Retardation Protein
  • In Vitro Precursor MicroRNA Processing Assays Using Drosophila Schneider-2 Cell Lysates
  • Downregulation of Human Cdc6 Protein Using a Lentivirus RNA Interference Expression Vector
  • Gene Silencing In Vitro and In Vivo Using Intronic MicroRNAs
  • Isolation and Identification of Gene-Specific MicroRNAs
  • Transgene-Like Animal Models Using Intronic MicroRNAs
  • Evolution of MicroRNAs
  • Perspectives.