Plant Proteomics Methods and Protocols /

Detalles Bibliográficos
Autor Corporativo: SpringerLink (Online service)
Otros Autores: Thiellement, Hervé. (Editor )
Formato: eBook
Lenguaje:English
Publicado: Totowa, NJ : Humana Press : Imprint: Humana, 2007.
Edición:1st ed. 2007.
Colección:Methods in Molecular Biology, 355
Materias:
Acceso en línea:https://doi.org/10.1385/1597452270
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040 |a Sistema de Bibliotecas del Tecnológico de Costa Rica 
245 1 0 |a Plant Proteomics  |b Methods and Protocols /  |c edited by Hervé Thiellement. 
250 |a 1st ed. 2007. 
260 # # |a Totowa, NJ :  |b Humana Press :  |b Imprint: Humana,  |c 2007. 
300 |a 416 p. 135 illus.  |b online resource. 
336 |a text  |b txt  |2 rdacontent 
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338 |a online resource  |b cr  |2 rdacarrier 
490 1 |a Methods in Molecular Biology,  |v 355 
505 0 |a Total Protein Extraction with TCA-Acetone -- Phenol Extraction of Proteins for Proteomic Studies of Recalcitrant Plant Tissues -- Protein Extraction from Cereal Seeds -- Protein Extraction from Xylem and Phloem Sap -- Protein Extraction from Woody Plants -- Isolation of Chloroplast Proteins from Arabidopsis thaliana for Proteome Analysis -- Isolation and Subfractionation of Plant Mitochondria for Proteomic Analysis -- Extraction of Nuclear Proteins from Root Meristematic Cells -- Extraction of Nuclear Proteins -- Isolation of Cell Wall Proteins from Medicago sativa Stems -- Plant Plasma Membrane Protein Extraction and Solubilization for Proteomic Analysis -- Detergents and Chaotropes for Protein Solubilization before Two-Dimensional Electrophoresis -- Two-Dimensional Electrophoresis for Plant Proteomics -- Visible and Fluorescent Staining of Two-Dimensional Gels -- Two-Dimensional Differential In-Gel Electrophoresis (DIGE) of Leaf and Roots of Lycopersicon esculentum -- Quantitative Analysis of 2D Gels -- Multivariate Data Analysis of Proteome Data -- Edman Sequencing of Proteins from 2D Gels -- Peptide Mass Fingerprinting -- Protein Identification Using Nano Liquid Chromatography-Tandem Mass Spectrometry -- Two-Dimensional Nanoflow Liquid Chromatography-Tandem Mass Spectrometry of Proteins Extracted from Rice Leaves and Roots -- Separation, Identification, and Profiling of Membrane Proteins by GFC/IEC/SDS-PAGE and MALDI TOF MS -- The PROTICdb Database for 2-DE Proteomics -- Identification of Phosphorylated Proteins -- Plant Proteomics and Glycosylation -- Blue-Native Gel Electrophoresis for the Characterization of Protein Complexes in Plants -- Electroelution of Intact Proteins from SDS-PAGE Gels and Their Subsequent MALDI-TOF MS Analysis -- Generation of Plant Protein Microarrays and Investigation of Antigen-Antibody Interactions -- Phosphorylation Studies Using Plant Protein Microarrays. 
650 0 |a Plant science. 
650 0 |a Botany. 
650 1 4 |a Plant Sciences. 
700 1 |a Thiellement, Hervé.  |e editor. 
710 2 |a SpringerLink (Online service) 
773 0 |t Springer eBooks 
856 4 0 |u https://doi.org/10.1385/1597452270