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02923nam a22003015i 4500 |
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978-1-59745-227-4 |
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20191027052048.0 |
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cr nn 008mamaa |
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100301s2007 xxu| s |||| 0|eng d |
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|a 9781597452274
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024 |
7 |
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|a 10.1385/1597452270
|2 doi
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|a Sistema de Bibliotecas del Tecnológico de Costa Rica
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245 |
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|a Plant Proteomics
|b Methods and Protocols /
|c edited by Hervé Thiellement.
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|a 1st ed. 2007.
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|a Totowa, NJ :
|b Humana Press :
|b Imprint: Humana,
|c 2007.
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|a 416 p. 135 illus.
|b online resource.
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336 |
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|a text
|b txt
|2 rdacontent
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337 |
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|a computer
|b c
|2 rdamedia
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338 |
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|a online resource
|b cr
|2 rdacarrier
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490 |
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|a Methods in Molecular Biology,
|v 355
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|a Total Protein Extraction with TCA-Acetone -- Phenol Extraction of Proteins for Proteomic Studies of Recalcitrant Plant Tissues -- Protein Extraction from Cereal Seeds -- Protein Extraction from Xylem and Phloem Sap -- Protein Extraction from Woody Plants -- Isolation of Chloroplast Proteins from Arabidopsis thaliana for Proteome Analysis -- Isolation and Subfractionation of Plant Mitochondria for Proteomic Analysis -- Extraction of Nuclear Proteins from Root Meristematic Cells -- Extraction of Nuclear Proteins -- Isolation of Cell Wall Proteins from Medicago sativa Stems -- Plant Plasma Membrane Protein Extraction and Solubilization for Proteomic Analysis -- Detergents and Chaotropes for Protein Solubilization before Two-Dimensional Electrophoresis -- Two-Dimensional Electrophoresis for Plant Proteomics -- Visible and Fluorescent Staining of Two-Dimensional Gels -- Two-Dimensional Differential In-Gel Electrophoresis (DIGE) of Leaf and Roots of Lycopersicon esculentum -- Quantitative Analysis of 2D Gels -- Multivariate Data Analysis of Proteome Data -- Edman Sequencing of Proteins from 2D Gels -- Peptide Mass Fingerprinting -- Protein Identification Using Nano Liquid Chromatography-Tandem Mass Spectrometry -- Two-Dimensional Nanoflow Liquid Chromatography-Tandem Mass Spectrometry of Proteins Extracted from Rice Leaves and Roots -- Separation, Identification, and Profiling of Membrane Proteins by GFC/IEC/SDS-PAGE and MALDI TOF MS -- The PROTICdb Database for 2-DE Proteomics -- Identification of Phosphorylated Proteins -- Plant Proteomics and Glycosylation -- Blue-Native Gel Electrophoresis for the Characterization of Protein Complexes in Plants -- Electroelution of Intact Proteins from SDS-PAGE Gels and Their Subsequent MALDI-TOF MS Analysis -- Generation of Plant Protein Microarrays and Investigation of Antigen-Antibody Interactions -- Phosphorylation Studies Using Plant Protein Microarrays.
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650 |
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|a Plant science.
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650 |
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|a Botany.
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650 |
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|a Plant Sciences.
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700 |
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|a Thiellement, Hervé.
|e editor.
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710 |
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|a SpringerLink (Online service)
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|t Springer eBooks
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856 |
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|u https://doi.org/10.1385/1597452270
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