Plant Proteomics Methods and Protocols /
| Corporate Author: | |
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| Other Authors: | |
| Format: | eBook |
| Language: | English |
| Published: |
Totowa, NJ :
Humana Press : Imprint: Humana,
2007.
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| Edition: | 1st ed. 2007. |
| Series: | Methods in Molecular Biology,
355 |
| Subjects: | |
| Online Access: | https://doi.org/10.1385/1597452270 |
Table of Contents:
- Total Protein Extraction with TCA-Acetone
- Phenol Extraction of Proteins for Proteomic Studies of Recalcitrant Plant Tissues
- Protein Extraction from Cereal Seeds
- Protein Extraction from Xylem and Phloem Sap
- Protein Extraction from Woody Plants
- Isolation of Chloroplast Proteins from Arabidopsis thaliana for Proteome Analysis
- Isolation and Subfractionation of Plant Mitochondria for Proteomic Analysis
- Extraction of Nuclear Proteins from Root Meristematic Cells
- Extraction of Nuclear Proteins
- Isolation of Cell Wall Proteins from Medicago sativa Stems
- Plant Plasma Membrane Protein Extraction and Solubilization for Proteomic Analysis
- Detergents and Chaotropes for Protein Solubilization before Two-Dimensional Electrophoresis
- Two-Dimensional Electrophoresis for Plant Proteomics
- Visible and Fluorescent Staining of Two-Dimensional Gels
- Two-Dimensional Differential In-Gel Electrophoresis (DIGE) of Leaf and Roots of Lycopersicon esculentum
- Quantitative Analysis of 2D Gels
- Multivariate Data Analysis of Proteome Data
- Edman Sequencing of Proteins from 2D Gels
- Peptide Mass Fingerprinting
- Protein Identification Using Nano Liquid Chromatography-Tandem Mass Spectrometry
- Two-Dimensional Nanoflow Liquid Chromatography-Tandem Mass Spectrometry of Proteins Extracted from Rice Leaves and Roots
- Separation, Identification, and Profiling of Membrane Proteins by GFC/IEC/SDS-PAGE and MALDI TOF MS
- The PROTICdb Database for 2-DE Proteomics
- Identification of Phosphorylated Proteins
- Plant Proteomics and Glycosylation
- Blue-Native Gel Electrophoresis for the Characterization of Protein Complexes in Plants
- Electroelution of Intact Proteins from SDS-PAGE Gels and Their Subsequent MALDI-TOF MS Analysis
- Generation of Plant Protein Microarrays and Investigation of Antigen-Antibody Interactions
- Phosphorylation Studies Using Plant Protein Microarrays.