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02758nam a22003855i 4500 |
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978-1-59745-229-8 |
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20191027032527.0 |
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cr nn 008mamaa |
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100301s2007 xxu| s |||| 0|eng d |
| 020 |
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|a 9781597452298
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| 024 |
7 |
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|a 10.1385/1597452297
|2 doi
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| 040 |
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|a Sistema de Bibliotecas del Tecnológico de Costa Rica
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| 245 |
1 |
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|a Protocols for Nucleic Acid Analysis by Nonradioactive Probes
|c edited by Elena Hilario, John F. MacKay.
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| 250 |
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|a 2nd ed. 2007.
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| 260 |
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|a Totowa, NJ :
|b Humana Press :
|b Imprint: Humana,
|c 2007.
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| 300 |
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|a 321 p. 66 illus., 5 illus. in color.
|b online resource.
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| 336 |
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|a text
|b txt
|2 rdacontent
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| 337 |
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|a computer
|b c
|2 rdamedia
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| 338 |
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|a online resource
|b cr
|2 rdacarrier
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| 490 |
1 |
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|a Methods in Molecular Biology,
|v 353
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| 505 |
0 |
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|a Nucleic Acid Extractions -- Genomic DNA Isolation From Different Biological Materials -- Extraction of Plant RNA -- Indirect Detection -- Overview of Hybridization and Detection Techniques -- Checkerboard DNA-DNA Hybridization Technology Using Digoxigenin Detection -- Nonradioactive Northern and Southern Analyses From Plant Samples -- Screening a BAC Library With Nonradioactive Overlapping Oligonucleotide (Overgo) Probes -- Direct In-Gel Hybridization of DNA With Digoxigenin-Labeled Probes -- In Situ Hybridization of Termite Microbes -- Fluorescent Labeling and Detection -- RNA Electrophoretic Mobility Shift Assay Using a Fluorescent DNA Sequencer -- Comparative Quantitation of mRNA Expression in the Central Nervous System Using Fluorescence In Situ Hybridization -- Visualization of Gene Expression by Fluorescent Multiplex Reverse Transcriptase-PCR Amplification -- Fluorescence In Situ Hybridization for the Identification of Environmental Microbes -- Kinetic (“Real-Time”) PCR -- to Kinetic (Real-Time) PCR -- Validation of Short Interfering RNA Knockdowns by Quantitative Real-Time PCR -- Real-Time Quantitative PCR as an Alternative to Southern Blot or Fluorescence In Situ Hybridization for Detection of Gene Copy Number Changes -- Design and Work-Up of a New Molecular Diagnostic Assay Based on Real-Time PCR -- Real-Time PCR Fluorescent Chemistries -- Microarrays -- Microarrays -- Oligonucleotide Microarrays for the Study of Coastal Microbial Communities.
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| 650 |
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0 |
|a Biochemistry.
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| 650 |
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0 |
|a Human genetics.
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| 650 |
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0 |
|a Cell biology.
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| 650 |
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0 |
|a Plant science.
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| 650 |
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0 |
|a Botany.
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| 650 |
1 |
4 |
|a Biochemistry, general.
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| 650 |
2 |
4 |
|a Human Genetics.
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| 650 |
2 |
4 |
|a Cell Biology.
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| 650 |
2 |
4 |
|a Plant Sciences.
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| 700 |
1 |
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|a Hilario, Elena.
|e editor.
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| 700 |
1 |
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|a MacKay, John F.
|e editor.
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| 710 |
2 |
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|a SpringerLink (Online service)
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| 773 |
0 |
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|t Springer eBooks
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| 856 |
4 |
0 |
|u https://doi.org/10.1385/1597452297
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