Protocols for Nucleic Acid Analysis by Nonradioactive Probes
| Autor Corporativo: | |
|---|---|
| Otros Autores: | , |
| Formato: | eBook |
| Lenguaje: | English |
| Publicado: |
Totowa, NJ :
Humana Press : Imprint: Humana,
2007.
|
| Edición: | 2nd ed. 2007. |
| Colección: | Methods in Molecular Biology,
353 |
| Materias: | |
| Acceso en línea: | https://doi.org/10.1385/1597452297 |
Tabla de Contenidos:
- Nucleic Acid Extractions
- Genomic DNA Isolation From Different Biological Materials
- Extraction of Plant RNA
- Indirect Detection
- Overview of Hybridization and Detection Techniques
- Checkerboard DNA-DNA Hybridization Technology Using Digoxigenin Detection
- Nonradioactive Northern and Southern Analyses From Plant Samples
- Screening a BAC Library With Nonradioactive Overlapping Oligonucleotide (Overgo) Probes
- Direct In-Gel Hybridization of DNA With Digoxigenin-Labeled Probes
- In Situ Hybridization of Termite Microbes
- Fluorescent Labeling and Detection
- RNA Electrophoretic Mobility Shift Assay Using a Fluorescent DNA Sequencer
- Comparative Quantitation of mRNA Expression in the Central Nervous System Using Fluorescence In Situ Hybridization
- Visualization of Gene Expression by Fluorescent Multiplex Reverse Transcriptase-PCR Amplification
- Fluorescence In Situ Hybridization for the Identification of Environmental Microbes
- Kinetic (“Real-Time”) PCR
- to Kinetic (Real-Time) PCR
- Validation of Short Interfering RNA Knockdowns by Quantitative Real-Time PCR
- Real-Time Quantitative PCR as an Alternative to Southern Blot or Fluorescence In Situ Hybridization for Detection of Gene Copy Number Changes
- Design and Work-Up of a New Molecular Diagnostic Assay Based on Real-Time PCR
- Real-Time PCR Fluorescent Chemistries
- Microarrays
- Microarrays
- Oligonucleotide Microarrays for the Study of Coastal Microbial Communities.