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02839nam a22002895i 4500 |
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978-1-59745-249-6 |
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20191027002939.0 |
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cr nn 008mamaa |
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100301s2008 xxu| s |||| 0|eng d |
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|a 9781597452496
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|a 10.1007/978-1-59745-249-6
|2 doi
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|a Sistema de Bibliotecas del Tecnológico de Costa Rica
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| 245 |
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|a Wnt Signaling
|b Volume 1: Pathway Methods and Mammalian Models /
|c edited by Elizabeth Vincan.
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| 250 |
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|a 1st ed. 2008.
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|a Totowa, NJ :
|b Humana Press :
|b Imprint: Humana,
|c 2008.
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| 300 |
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|a XVI, 336 p. 76 illus., 2 illus. in color.
|b online resource.
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| 336 |
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|a text
|b txt
|2 rdacontent
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|a computer
|b c
|2 rdamedia
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|a online resource
|b cr
|2 rdacarrier
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| 490 |
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|a Methods in Molecular Biology,
|v 468
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|a Wnt Signaling Pathway Methods -- The Canonical Wnt/?-Catenin Signalling Pathway -- Isolation and Application of Bioactive Wnt Proteins -- Purification and Wnt-Inhibitory Activities of Secreted Frizzled-Related Proteins -- Measuring GSK3 Expression and Activity in Cells -- Inhibition of Glycogen Synthase Kinase-3 -- Detection of Cytoplasmic and Nuclear Localization of Adenomatous Polyposis Coli (APC) Protein in Cells -- Detection of ?-Catenin Localization by Immunohistochemistry -- Assaying ?-Catenin/TCF Transcription with ?-Catenin/TCF Transcription-Based Reporter Constructs -- Native Promoter Reporters Validate Transcriptional Targets -- ?-Catenin-Independent Wnt Pathways: Signals, Core Proteins, and Effectors -- Image Analysis of Calcium Release Dynamics -- Detecting PKC Phosphorylation as Part of the Wnt/Calcium Pathway in Cutaneous Melanoma -- Measuring CamKII Activity in Xenopus Embryos as a Read-out for Non-canonical Wnt Signaling -- Analysis of Wnt7a-Stimulated JNK Activity and cJun Phosphorylation in Non-Small Cell Lung Cancer Cells -- ROCK Enzymatic Assay -- Detection of Planar Polarity Proteins in Mammalian Cochlea -- Mammalian Model Systems for Wnt/FZD Function -- Proteomic Analyses of Protein Complexes in the Wnt Pathway -- In Situ Hybridization to Evaluate the Expression of Wnt and Frizzled Genes in Mammalian Tissues -- Assaying Wnt5A-Mediated Invasion in Melanoma Cells -- Coculture Methodologies for the Study of Wnt Signals -- Analysis of Wnt/FZD-Mediated Signalling in a Cell Line Model of Colorectal Cancer Morphogenesis -- Analysing Tissue and Gene Function in Intestinal Organ Culture -- Genetics of Wnt Signaling During Early Mammalian Development -- Tissue-Specific Transgenic, Conditional Knockout and Knock-In Mice of Genes in the Canonical Wnt Signaling Pathway.
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| 650 |
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|a Cell biology.
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| 650 |
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|a Cell Biology.
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| 700 |
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|a Vincan, Elizabeth.
|e editor.
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| 710 |
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|a SpringerLink (Online service)
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|t Springer eBooks
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| 856 |
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|u https://doi.org/10.1007/978-1-59745-249-6
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