Wnt Signaling Volume 1: Pathway Methods and Mammalian Models /
| Autor Corporativo: | |
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| Otros Autores: | |
| Formato: | eBook |
| Lenguaje: | English |
| Publicado: |
Totowa, NJ :
Humana Press : Imprint: Humana,
2008.
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| Edición: | 1st ed. 2008. |
| Colección: | Methods in Molecular Biology,
468 |
| Materias: | |
| Acceso en línea: | https://doi.org/10.1007/978-1-59745-249-6 |
Tabla de Contenidos:
- Wnt Signaling Pathway Methods
- The Canonical Wnt/?-Catenin Signalling Pathway
- Isolation and Application of Bioactive Wnt Proteins
- Purification and Wnt-Inhibitory Activities of Secreted Frizzled-Related Proteins
- Measuring GSK3 Expression and Activity in Cells
- Inhibition of Glycogen Synthase Kinase-3
- Detection of Cytoplasmic and Nuclear Localization of Adenomatous Polyposis Coli (APC) Protein in Cells
- Detection of ?-Catenin Localization by Immunohistochemistry
- Assaying ?-Catenin/TCF Transcription with ?-Catenin/TCF Transcription-Based Reporter Constructs
- Native Promoter Reporters Validate Transcriptional Targets
- ?-Catenin-Independent Wnt Pathways: Signals, Core Proteins, and Effectors
- Image Analysis of Calcium Release Dynamics
- Detecting PKC Phosphorylation as Part of the Wnt/Calcium Pathway in Cutaneous Melanoma
- Measuring CamKII Activity in Xenopus Embryos as a Read-out for Non-canonical Wnt Signaling
- Analysis of Wnt7a-Stimulated JNK Activity and cJun Phosphorylation in Non-Small Cell Lung Cancer Cells
- ROCK Enzymatic Assay
- Detection of Planar Polarity Proteins in Mammalian Cochlea
- Mammalian Model Systems for Wnt/FZD Function
- Proteomic Analyses of Protein Complexes in the Wnt Pathway
- In Situ Hybridization to Evaluate the Expression of Wnt and Frizzled Genes in Mammalian Tissues
- Assaying Wnt5A-Mediated Invasion in Melanoma Cells
- Coculture Methodologies for the Study of Wnt Signals
- Analysis of Wnt/FZD-Mediated Signalling in a Cell Line Model of Colorectal Cancer Morphogenesis
- Analysing Tissue and Gene Function in Intestinal Organ Culture
- Genetics of Wnt Signaling During Early Mammalian Development
- Tissue-Specific Transgenic, Conditional Knockout and Knock-In Mice of Genes in the Canonical Wnt Signaling Pathway.