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04040nam a22003495i 4500 |
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978-1-59745-321-9 |
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20191027151119.0 |
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cr nn 008mamaa |
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100301s2008 xxu| s |||| 0|eng d |
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|a 9781597453219
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7 |
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|a 10.1007/978-1-59745-321-9
|2 doi
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| 040 |
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|a Sistema de Bibliotecas del Tecnológico de Costa Rica
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| 245 |
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|a Microbial Gene Essentiality: Protocols and Bioinformatics
|c edited by Andrei L. Osterman, Svetlana Y. Gerdes.
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| 250 |
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|a 1st ed. 2008.
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| 260 |
# |
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|a Totowa, NJ :
|b Humana Press :
|b Imprint: Humana,
|c 2008.
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| 300 |
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|a XIV, 486 p.
|b online resource.
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| 336 |
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|a text
|b txt
|2 rdacontent
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| 337 |
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|a computer
|b c
|2 rdamedia
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| 338 |
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|a online resource
|b cr
|2 rdacarrier
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| 490 |
1 |
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|a Methods in Molecular Biology,
|v 416
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| 505 |
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|a Overview of Whole-Genome Essentiality Analysis -- Experimental Protocols -- Transposon-Based Strategies for the Identification of Essential Bacterial Genes -- Identification and Analysis of Essential Genes in Haemophilus influenzae -- Transposon Site Hybridization in Mycobacterium tuberculosis -- Essential Genes in the Infection Model of Pseudomonas aeruginosa PCR-Based Signature-Tagged Mutagenesis -- Whole-Genome Detection of Conditionally Essential and Dispensable Genes in Escherichia coli via Genetic Footprinting -- Generating a Collection of Insertion Mutations in the Staphylococcus aureus Genome Using bursa aurealis -- Multipurpose Transposon Insertion Libraries for Large-Scale Analysis of Gene Function in Yeast -- How to Make a Defined Near-Saturation Mutant Library. Case 1: Pseudomonas aeruginosa PAO1 -- Comparing Insertion Libraries in Two Pseudomonas aeruginosa Strains to Assess Gene Essentiality -- The Construction of Systematic In-Frame, Single-Gene Knockout Mutant Collection in Escherichia coli K-12 -- The Applications of Systematic In-Frame, Single-Gene Knockout Mutant Collection of Escherichia coli K-12 -- A Novel, Simple, High-Throughput Method for Isolation of Genome-Wide Transposon Insertion Mutants of Escherichia coli K-12 -- High-Throughput Creation of a Whole-Genome Collection of Yeast Knockout Strains -- Analysis of Genetic Interactions on a Genome-Wide Scale in Budding Yeast: Diploid-Based Synthetic Lethality Analysis by Microarray -- Scarless Engineering of the Escherichia coli Genome -- Minimization of the Escherichia coli Genome Using the Tn5-Targeted Cre/loxP Excision System -- Construction of Long Chromosomal Deletion Mutants of Escherichia coli and Minimization of the Genome -- Identification of Essential Genes in Staphylococcus aureus by Construction and Screening of Conditional Mutant Library -- Techniques for the Isolation and Use of Conditionally Expressed Antisense RNA to Achieve Essential Gene Knockdowns in Staphylococcus aureus -- of Conditional Lethal Amber Mutations in Escherichia coli -- Bioinformatics -- Statistical Methods for Building Random Transposon Mutagenesis Libraries -- Statistical Evaluation of Genetic Footprinting Data -- Modeling Competitive Outgrowth of Mutant Populations: Why Do Essentiality Screens Yield Divergent Results? -- Statistical Analysis of Fitness Data Determined by TAG Hybridization on Microarrays -- Profiling of Escherichia coli Chromosome Database -- Gene Essentiality Analysis Based on DEG, a Database of Essential Genes -- Detection of Essential Genes in Streptococcus pneumoniae Using Bioinformatics and Allelic Replacement Mutagenesis -- Design and Application of Genome-Scale Reconstructed Metabolic Models -- Predicting Gene Essentiality Using Genome-Scale in Silico Models -- Comparative Approach to Analysis of Gene Essentiality.
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| 650 |
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0 |
|a Biochemistry.
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| 650 |
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0 |
|a Cell biology.
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| 650 |
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0 |
|a Human genetics.
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| 650 |
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4 |
|a Biochemistry, general.
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| 650 |
2 |
4 |
|a Cell Biology.
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| 650 |
2 |
4 |
|a Human Genetics.
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| 700 |
1 |
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|a Osterman, Andrei L.
|e editor.
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| 700 |
1 |
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|a Gerdes, Svetlana Y.
|e editor.
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| 710 |
2 |
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|a SpringerLink (Online service)
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| 773 |
0 |
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|t Springer eBooks
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| 856 |
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0 |
|u https://doi.org/10.1007/978-1-59745-321-9
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