Transgenic Wheat, Barley and Oats Production and Characterization Protocols /

Bibliographic Details
Corporate Author: SpringerLink (Online service)
Other Authors: Jones, Huw D. (Editor), Shewry, Peter R. (Editor)
Format: eBook
Language:English
Published: Totowa, NJ : Humana Press : Imprint: Humana, 2009.
Edition:1st ed. 2009.
Series:Methods in Molecular Biology, 478
Subjects:
Online Access:https://doi.org/10.1007/978-1-59745-379-0
Table of Contents:
  • Transgenic Wheat, Barley and Oats: Production and Characterization
  • Transformation and Regeneration
  • Selection of Transformed Plants
  • Reporter Genes
  • Biolistics Transformation of Wheat
  • Agrobacterium-Mediated Transformation of Bread and Durum Wheat Using Freshly Isolated Immature Embryos
  • Floral Transformation of Wheat
  • Highly Efficient Agrobacterium-Mediated Transformation of Wheat Via In Planta Inoculation
  • Barley Transformation Using Biolistic Techniques
  • Barley Transformation Using Agrobacterium-Mediated Techniques
  • Transformation of Oats and Its Application to Improving Osmotic Stress Tolerance
  • Gene and Protein Expression
  • Promoter Sequences for Defining Transgene Expression
  • Down-Regulation of Gene Expression by RNA-Induced Gene Silencing
  • Characterisation of Transgenic Plants
  • Gene Insertion Patterns and Sites
  • Fluorescent In Situ Hybridization to Detect Transgene Integration into Plant Genomes
  • Establishing Substantial Equivalence: Transcriptomics
  • Establishing Substantial Equivalence: Proteomics
  • Establishing Substantial Equivalence: Metabolomics
  • Design and Management of Field Trials of Transgenic Cereals
  • GM Risk Assessment
  • Conclusions
  • Transgenic Wheat, Barley and Oats: Future Prospects.