Macrophages and Dendritic Cells Methods and Protocols /

Detalles Bibliográficos
Autor Corporativo: SpringerLink (Online service)
Otros Autores: Reiner, Neil E. (Editor )
Formato: eBook
Lenguaje:English
Publicado: Totowa, NJ : Humana Press : Imprint: Humana, 2009.
Edición:1st ed. 2009.
Colección:Methods in Molecular Biology, 531
Materias:
Acceso en línea:https://doi.org/10.1007/978-1-59745-396-7
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245 1 0 |a Macrophages and Dendritic Cells  |b Methods and Protocols /  |c edited by Neil E. Reiner. 
250 |a 1st ed. 2009. 
260 # # |a Totowa, NJ :  |b Humana Press :  |b Imprint: Humana,  |c 2009. 
300 |a XII, 368 p.  |b online resource. 
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490 1 |a Methods in Molecular Biology,  |v 531 
505 0 |a Generation of Retroviral Macrophage cDNA Expression Libraries and Functional Screening for Surface Receptors -- Generation of Murine Growth Factor-Dependent Long-Term Dendritic Cell Lines to Investigate Host-Parasite Interactions -- Macrophage Activation: Classical Vs. Alternative -- Analysis of Macrophage Phagocytosis: Quantitative Assays of Phagosome Formation and Maturation Using High-Throughput Fluorescence Microscopy -- Detection of Activated Rab7 GTPase with an Immobilized RILP Probe -- Probing In Vivo Origins of Mononuclear Phagocytes by Conditional Ablation and Reconstitution -- Multivalent Protein Probes for the Identification and Characterization of Cognate Cellular Ligands for Myeloid Cell Surface Receptors -- Exploiting Fc Chimaeric Proteins for the Identification of Ligands Specific for the Mannose Receptor -- The Expression of Exogenous Genes in Macrophages: Obstacles and Opportunities -- Genetic Modification of Murine Dendritic Cells by RNA Transfection -- Recording Phagosome Maturation Through the Real-Time, Spectrofluorometric Measurement of Hydrolytic Activities -- Derivation and Characterization of Murine Alternatively Activated (M2) Macrophages -- Isolation and Culture of Human Hematopoietic Progenitors for Studies of Dendritic Cell Biology -- Generation, Culture and Flow-Cytometric Characterization of Primary Mouse Macrophages -- Analyzing Classical and Alternative Macrophage Activation in Macrophage/Neutrophil-Specific IL-4 Receptor-Alpha-Deficient Mice -- Analysis of Matrix Metalloproteinase Secretion by Macrophages -- Identification of Pathogen Receptors on Dendritic Cells to Understand their Function and to Identify New Drug Targets -- Stable Lentiviral Vector-Mediated Gene Silencing in Human Monocytic Cell Lines -- Use of Fluorescent Probes to Detect Lipid Signaling Intermediates in Macrophages -- Large-Scale Phagosome Preparation -- Coordinate Regulation of Sphingosine Kinase and Actin Dynamics. 
650 0 |a Immunology. 
650 0 |a Cell culture. 
650 1 4 |a Immunology. 
650 2 4 |a Cell Culture. 
700 1 |a Reiner, Neil E.  |e editor. 
710 2 |a SpringerLink (Online service) 
773 0 |t Springer eBooks 
856 4 0 |u https://doi.org/10.1007/978-1-59745-396-7