Macrophages and Dendritic Cells Methods and Protocols /

Detalles Bibliográficos
Autor Corporativo: SpringerLink (Online service)
Otros Autores: Reiner, Neil E. (Editor )
Formato: eBook
Lenguaje:English
Publicado: Totowa, NJ : Humana Press : Imprint: Humana, 2009.
Edición:1st ed. 2009.
Colección:Methods in Molecular Biology, 531
Materias:
Acceso en línea:https://doi.org/10.1007/978-1-59745-396-7
Tabla de Contenidos:
  • Generation of Retroviral Macrophage cDNA Expression Libraries and Functional Screening for Surface Receptors
  • Generation of Murine Growth Factor-Dependent Long-Term Dendritic Cell Lines to Investigate Host-Parasite Interactions
  • Macrophage Activation: Classical Vs. Alternative
  • Analysis of Macrophage Phagocytosis: Quantitative Assays of Phagosome Formation and Maturation Using High-Throughput Fluorescence Microscopy
  • Detection of Activated Rab7 GTPase with an Immobilized RILP Probe
  • Probing In Vivo Origins of Mononuclear Phagocytes by Conditional Ablation and Reconstitution
  • Multivalent Protein Probes for the Identification and Characterization of Cognate Cellular Ligands for Myeloid Cell Surface Receptors
  • Exploiting Fc Chimaeric Proteins for the Identification of Ligands Specific for the Mannose Receptor
  • The Expression of Exogenous Genes in Macrophages: Obstacles and Opportunities
  • Genetic Modification of Murine Dendritic Cells by RNA Transfection
  • Recording Phagosome Maturation Through the Real-Time, Spectrofluorometric Measurement of Hydrolytic Activities
  • Derivation and Characterization of Murine Alternatively Activated (M2) Macrophages
  • Isolation and Culture of Human Hematopoietic Progenitors for Studies of Dendritic Cell Biology
  • Generation, Culture and Flow-Cytometric Characterization of Primary Mouse Macrophages
  • Analyzing Classical and Alternative Macrophage Activation in Macrophage/Neutrophil-Specific IL-4 Receptor-Alpha-Deficient Mice
  • Analysis of Matrix Metalloproteinase Secretion by Macrophages
  • Identification of Pathogen Receptors on Dendritic Cells to Understand their Function and to Identify New Drug Targets
  • Stable Lentiviral Vector-Mediated Gene Silencing in Human Monocytic Cell Lines
  • Use of Fluorescent Probes to Detect Lipid Signaling Intermediates in Macrophages
  • Large-Scale Phagosome Preparation
  • Coordinate Regulation of Sphingosine Kinase and Actin Dynamics.