The Nucleus Volume 1: Nuclei and Subnuclear Components /

Detalles Bibliográficos
Autor Corporativo: SpringerLink (Online service)
Otros Autores: Hancock, Ronald. (Editor )
Formato: eBook
Lenguaje:English
Publicado: Totowa, NJ : Humana Press : Imprint: Humana, 2008.
Edición:1st ed. 2008.
Colección:Methods in Molecular Biology, 463
Materias:
Acceso en línea:https://doi.org/10.1007/978-1-59745-406-3
Tabla de Contenidos:
  • The Intranuclear Environment
  • The Intranuclear Environment
  • Isolation of Nuclei
  • Purification of Nuclei and Preparation of Nuclear Envelopes from Skeletal Muscle
  • Isolation of Highly Purified Yeast Nuclei for Nuclease Mapping of Chromatin Structure
  • Working with Oocyte Nuclei: Cytological Preparations of Active Chromatin and Nuclear Bodies from Amphibian Germinal Vesicles
  • Preparation of Arabidopsis Nuclei and Nucleoli
  • High-Yield Isolation and Subcellular Proteomic Characterization of Nuclear and Subnuclear Structures from Trypanosomes
  • Methods for Studying the Nuclei and Chromosomes of Dinoflagellates
  • The Nucleolus
  • Isolation of Nucleoli from Ehrlich Ascites Tumor Cells and Dynamics of Nascent RNA within Isolated Nucleoli
  • Time-lapse Microscopy and Fluorescence Resonance Energy Transfer to Analyze the Dynamics and Interactions of Nucleolar Proteins in Living Cells
  • Three-Dimensional Reconstruction of Nucleolar Components by Electron Microscope Tomography
  • Intranuclear Structures
  • The Perinucleolar Compartment (PNC): Detection by Immunohistochemistry
  • Isolation of the Constitutive Heterochromatin from Mouse Liver Nuclei
  • Isolation of Pathology-Associated Intranuclear Inclusions
  • The Nuclear Ubiquitin–Proteasome System: Visualization of Proteasomes, Protein Aggregates, and Proteolysis in the Cell Nucleus
  • Interphase Chromosomes
  • Multicolor 3D Fluorescence In Situ Hybridization for Imaging Interphase Chromosomes
  • Fluorescent Transgenes to Study Interphase Chromosomes in Living Plants
  • Analysis of Telomeres and Telomerase
  • Combined Immunofluorescence, RNA Fluorescent In Situ Hybridization, and DNA Fluorescent In Situ Hybridization to Study Chromatin Changes, Transcriptional Activity, Nuclear Organization, and X-Chromosome Inactivation
  • Analysis of the Mobility of DNA Double-Strand Break-Containing Chromosome Domains in Living Mammalian Cells.