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02369nam a22003735i 4500 |
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978-1-59745-456-8 |
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20191027062312.0 |
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cr nn 008mamaa |
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100301s2007 xxu| s |||| 0|eng d |
| 020 |
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|a 9781597454568
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| 024 |
7 |
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|a 10.1007/978-1-59745-456-8
|2 doi
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| 040 |
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|a Sistema de Bibliotecas del Tecnológico de Costa Rica
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| 245 |
1 |
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|a Pichia Protocols
|c edited by James M. Cregg.
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| 250 |
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|a 2nd ed. 2007.
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| 260 |
# |
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|a Totowa, NJ :
|b Humana Press :
|b Imprint: Humana,
|c 2007.
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| 300 |
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|a XII, 268 p.
|b online resource.
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| 336 |
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|a text
|b txt
|2 rdacontent
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| 337 |
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|a computer
|b c
|2 rdamedia
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| 338 |
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|a online resource
|b cr
|2 rdacarrier
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| 490 |
1 |
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|a Methods in Molecular Biology,
|v 389
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|a Vectors and Strains for Expression -- DNA-Mediated Transformation -- Rational Design and Optimization of Fed-Batch and Continuous Fermentations -- Saturation of the Secretory Pathway by Overexpression of a Hookworm (Necator americanus) Protein (Na-ASP1) -- Purification of the N- and C-Terminal Subdomains of Recombinant Heavy Chain Fragment C of Botulinum Neurotoxin Serotype C -- Rapid Screening of Chromatography Resins for the Purification of Proteins -- Characterization of O-Linked Saccharides on Glycoproteins -- Modification of the N-Glycosylation Pathway to Produce Homogeneous, Human-Like Glycans Using GlycoSwitch Plasmids -- N-Linked Glycan Characterization of Heterologous Proteins -- Heavy Labeling of Recombinant Proteins -- Selenomethionine Labeling of Recombinant Proteins -- Selective Isotopic Labeling of Recombinant Proteins Using Amino Acid Auxotroph Strains -- Classical Genetics -- Identification of Pexophagy Genes by Restriction Enzyme-Mediated Integration -- Characterization of Protein-Protein Interactions -- Localization of Proteins and Organelles Using Fluorescence Microscopy -- Fluorescence Microscopy and Thin-Section Electron Microscopy.
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| 650 |
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|a Biotechnology.
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| 650 |
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|a Cell biology.
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| 650 |
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|a Microbial genetics.
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| 650 |
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|a Microbial genomics.
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| 650 |
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|a Microscopy.
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| 650 |
1 |
4 |
|a Biotechnology.
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| 650 |
2 |
4 |
|a Cell Biology.
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| 650 |
2 |
4 |
|a Microbial Genetics and Genomics.
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| 650 |
2 |
4 |
|a Biological Microscopy.
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| 700 |
1 |
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|a Cregg, James M.
|e editor.
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| 710 |
2 |
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|a SpringerLink (Online service)
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| 773 |
0 |
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|t Springer eBooks
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| 856 |
4 |
0 |
|u https://doi.org/10.1007/978-1-59745-456-8
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