Pichia Protocols
| Corporate Author: | |
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| Other Authors: | |
| Format: | eBook |
| Language: | English |
| Published: |
Totowa, NJ :
Humana Press : Imprint: Humana,
2007.
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| Edition: | 2nd ed. 2007. |
| Series: | Methods in Molecular Biology,
389 |
| Subjects: | |
| Online Access: | https://doi.org/10.1007/978-1-59745-456-8 |
Table of Contents:
- Vectors and Strains for Expression
- DNA-Mediated Transformation
- Rational Design and Optimization of Fed-Batch and Continuous Fermentations
- Saturation of the Secretory Pathway by Overexpression of a Hookworm (Necator americanus) Protein (Na-ASP1)
- Purification of the N- and C-Terminal Subdomains of Recombinant Heavy Chain Fragment C of Botulinum Neurotoxin Serotype C
- Rapid Screening of Chromatography Resins for the Purification of Proteins
- Characterization of O-Linked Saccharides on Glycoproteins
- Modification of the N-Glycosylation Pathway to Produce Homogeneous, Human-Like Glycans Using GlycoSwitch Plasmids
- N-Linked Glycan Characterization of Heterologous Proteins
- Heavy Labeling of Recombinant Proteins
- Selenomethionine Labeling of Recombinant Proteins
- Selective Isotopic Labeling of Recombinant Proteins Using Amino Acid Auxotroph Strains
- Classical Genetics
- Identification of Pexophagy Genes by Restriction Enzyme-Mediated Integration
- Characterization of Protein-Protein Interactions
- Localization of Proteins and Organelles Using Fluorescence Microscopy
- Fluorescence Microscopy and Thin-Section Electron Microscopy.