Neutrophil Methods and Protocols

Detalles Bibliográficos
Autor Corporativo: SpringerLink (Online service)
Otros Autores: Quinn, Mark T. (Editor ), DeLeo, Frank R. (Editor ), Bokoch, Gary M. (Editor )
Formato: eBook
Lenguaje:English
Publicado: Totowa, NJ : Humana Press : Imprint: Humana, 2007.
Edición:1st ed. 2007.
Colección:Methods in Molecular Biology, 412
Materias:
Acceso en línea:https://doi.org/10.1007/978-1-59745-467-4
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245 1 0 |a Neutrophil Methods and Protocols  |c edited by Mark T. Quinn, Frank R. DeLeo, Gary M. Bokoch. 
250 |a 1st ed. 2007. 
260 # # |a Totowa, NJ :  |b Humana Press :  |b Imprint: Humana,  |c 2007. 
300 |a XX, 537 p. 112 illus., 8 illus. in color.  |b online resource. 
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338 |a online resource  |b cr  |2 rdacarrier 
490 1 |a Methods in Molecular Biology,  |v 412 
505 0 |a Neutrophils: An Overview -- The Role of Neutrophils in the Immune System -- Neutrophil Isolation and Subcellular Fractionation -- Isolation of Human Neutrophils From Venous Blood -- Neutrophil Isolation From Nonhuman Species -- Subcellular Fractionation of Human Neutrophils and Analysis of Subcellular Markers -- Biochemistry, Biology, and Signal Transduction of Neutrophils -- Rac and Rap GTPase Activation Assays -- Measurement of Phospholipid Metabolism in Intact Neutrophils -- Analysis of Protein Phosphorylation in Human Neutrophils -- Mitogen-Activated Protein Kinase Assays -- Transduction of Proteins into Intact Neutrophils -- Optical Methods for the Measurement and Manipulation of Cytosolic Free Calcium in Neutrophils -- Analysis of Electrophysiological Properties and Responses of Neutrophils -- Analysis of Neutrophil Apoptosis -- Neutrophil Adhesion and Chemotaxis -- Optical and Fluorescence Detection of Neutrophil Integrin Activation -- Analysis of Neutrophil Polarization and Chemotaxis -- Quantifying and Localizing Actin-Free Barbed Ends in Neutrophils -- Measurement of Neutrophil Adhesion Under Conditions Mimicking Blood Flow -- Model Systems to Investigate Neutrophil Adhesion and Chemotaxis -- Neutrophil Phagocytosis and Bactericidal Activity -- Immunofluorescence and Confocal Microscopy of Neutrophils -- Assessment of Phagosome Formation and Maturation by Fluorescence Microscopy -- Analysis of Neutrophil Membrane Traffic During Phagocytosis -- Analysis of Neutrophil Bactericidal Activity -- A Skin Chamber Technique as a Human Model for Studies of Aseptic Inflammatory Reactions -- Nadph Oxidase and Production of Reactive Oxygen Species -- Measurement of Respiratory Burst Products Generated by Professional Phagocytes -- The K-562 Cell Model for Analysis of Neutrophil NADPH Oxidase Function -- Cell-Free Assays -- Immunoaffinity Purification of Human Phagocyte Flavocytochrome b and Analysis of Conformational Dynamics -- Analysis of Neutrophil Gene Expression and Transcription Factors -- Genome-Scale Transcript Analyses in Human Neutrophils -- Fast and Accurate Quantitative Analysis of Cytokine Gene Expression in Human Neutrophils by Reverse Transcription Real-Time PCR -- Detection of Intact Transcription Factors in Human Neutrophils -- Neutrophil Defects and Diagnosis -- Disorders of Neutrophil Function -- Diagnostic Assays for Chronic Granulomatous Disease and Other Neutrophil Disorders -- Diagnostic Assays for Myeloperoxidase Deficiency. 
650 0 |a Immunology. 
650 0 |a Cell biology. 
650 0 |a Human genetics. 
650 1 4 |a Immunology. 
650 2 4 |a Cell Biology. 
650 2 4 |a Human Genetics. 
700 1 |a Quinn, Mark T.  |e editor. 
700 1 |a DeLeo, Frank R.  |e editor. 
700 1 |a Bokoch, Gary M.  |e editor. 
710 2 |a SpringerLink (Online service) 
773 0 |t Springer eBooks 
856 4 0 |u https://doi.org/10.1007/978-1-59745-467-4