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03715nam a22003615i 4500 |
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978-1-59745-467-4 |
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20191026222039.0 |
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cr nn 008mamaa |
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100301s2007 xxu| s |||| 0|eng d |
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|a 9781597454674
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024 |
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|a 10.1007/978-1-59745-467-4
|2 doi
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|a Sistema de Bibliotecas del Tecnológico de Costa Rica
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245 |
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|a Neutrophil Methods and Protocols
|c edited by Mark T. Quinn, Frank R. DeLeo, Gary M. Bokoch.
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250 |
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|a 1st ed. 2007.
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260 |
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|a Totowa, NJ :
|b Humana Press :
|b Imprint: Humana,
|c 2007.
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300 |
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|a XX, 537 p. 112 illus., 8 illus. in color.
|b online resource.
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336 |
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|a text
|b txt
|2 rdacontent
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|a computer
|b c
|2 rdamedia
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|a online resource
|b cr
|2 rdacarrier
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490 |
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|a Methods in Molecular Biology,
|v 412
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|a Neutrophils: An Overview -- The Role of Neutrophils in the Immune System -- Neutrophil Isolation and Subcellular Fractionation -- Isolation of Human Neutrophils From Venous Blood -- Neutrophil Isolation From Nonhuman Species -- Subcellular Fractionation of Human Neutrophils and Analysis of Subcellular Markers -- Biochemistry, Biology, and Signal Transduction of Neutrophils -- Rac and Rap GTPase Activation Assays -- Measurement of Phospholipid Metabolism in Intact Neutrophils -- Analysis of Protein Phosphorylation in Human Neutrophils -- Mitogen-Activated Protein Kinase Assays -- Transduction of Proteins into Intact Neutrophils -- Optical Methods for the Measurement and Manipulation of Cytosolic Free Calcium in Neutrophils -- Analysis of Electrophysiological Properties and Responses of Neutrophils -- Analysis of Neutrophil Apoptosis -- Neutrophil Adhesion and Chemotaxis -- Optical and Fluorescence Detection of Neutrophil Integrin Activation -- Analysis of Neutrophil Polarization and Chemotaxis -- Quantifying and Localizing Actin-Free Barbed Ends in Neutrophils -- Measurement of Neutrophil Adhesion Under Conditions Mimicking Blood Flow -- Model Systems to Investigate Neutrophil Adhesion and Chemotaxis -- Neutrophil Phagocytosis and Bactericidal Activity -- Immunofluorescence and Confocal Microscopy of Neutrophils -- Assessment of Phagosome Formation and Maturation by Fluorescence Microscopy -- Analysis of Neutrophil Membrane Traffic During Phagocytosis -- Analysis of Neutrophil Bactericidal Activity -- A Skin Chamber Technique as a Human Model for Studies of Aseptic Inflammatory Reactions -- Nadph Oxidase and Production of Reactive Oxygen Species -- Measurement of Respiratory Burst Products Generated by Professional Phagocytes -- The K-562 Cell Model for Analysis of Neutrophil NADPH Oxidase Function -- Cell-Free Assays -- Immunoaffinity Purification of Human Phagocyte Flavocytochrome b and Analysis of Conformational Dynamics -- Analysis of Neutrophil Gene Expression and Transcription Factors -- Genome-Scale Transcript Analyses in Human Neutrophils -- Fast and Accurate Quantitative Analysis of Cytokine Gene Expression in Human Neutrophils by Reverse Transcription Real-Time PCR -- Detection of Intact Transcription Factors in Human Neutrophils -- Neutrophil Defects and Diagnosis -- Disorders of Neutrophil Function -- Diagnostic Assays for Chronic Granulomatous Disease and Other Neutrophil Disorders -- Diagnostic Assays for Myeloperoxidase Deficiency.
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650 |
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|a Immunology.
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650 |
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|a Cell biology.
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650 |
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0 |
|a Human genetics.
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650 |
1 |
4 |
|a Immunology.
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650 |
2 |
4 |
|a Cell Biology.
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650 |
2 |
4 |
|a Human Genetics.
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700 |
1 |
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|a Quinn, Mark T.
|e editor.
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700 |
1 |
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|a DeLeo, Frank R.
|e editor.
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700 |
1 |
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|a Bokoch, Gary M.
|e editor.
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710 |
2 |
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|a SpringerLink (Online service)
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773 |
0 |
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|t Springer eBooks
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856 |
4 |
0 |
|u https://doi.org/10.1007/978-1-59745-467-4
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