Neutrophil Methods and Protocols
| Autor Corporativo: | |
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| Otros Autores: | , , |
| Formato: | eBook |
| Lenguaje: | English |
| Publicado: |
Totowa, NJ :
Humana Press : Imprint: Humana,
2007.
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| Edición: | 1st ed. 2007. |
| Colección: | Methods in Molecular Biology,
412 |
| Materias: | |
| Acceso en línea: | https://doi.org/10.1007/978-1-59745-467-4 |
Tabla de Contenidos:
- Neutrophils: An Overview
- The Role of Neutrophils in the Immune System
- Neutrophil Isolation and Subcellular Fractionation
- Isolation of Human Neutrophils From Venous Blood
- Neutrophil Isolation From Nonhuman Species
- Subcellular Fractionation of Human Neutrophils and Analysis of Subcellular Markers
- Biochemistry, Biology, and Signal Transduction of Neutrophils
- Rac and Rap GTPase Activation Assays
- Measurement of Phospholipid Metabolism in Intact Neutrophils
- Analysis of Protein Phosphorylation in Human Neutrophils
- Mitogen-Activated Protein Kinase Assays
- Transduction of Proteins into Intact Neutrophils
- Optical Methods for the Measurement and Manipulation of Cytosolic Free Calcium in Neutrophils
- Analysis of Electrophysiological Properties and Responses of Neutrophils
- Analysis of Neutrophil Apoptosis
- Neutrophil Adhesion and Chemotaxis
- Optical and Fluorescence Detection of Neutrophil Integrin Activation
- Analysis of Neutrophil Polarization and Chemotaxis
- Quantifying and Localizing Actin-Free Barbed Ends in Neutrophils
- Measurement of Neutrophil Adhesion Under Conditions Mimicking Blood Flow
- Model Systems to Investigate Neutrophil Adhesion and Chemotaxis
- Neutrophil Phagocytosis and Bactericidal Activity
- Immunofluorescence and Confocal Microscopy of Neutrophils
- Assessment of Phagosome Formation and Maturation by Fluorescence Microscopy
- Analysis of Neutrophil Membrane Traffic During Phagocytosis
- Analysis of Neutrophil Bactericidal Activity
- A Skin Chamber Technique as a Human Model for Studies of Aseptic Inflammatory Reactions
- Nadph Oxidase and Production of Reactive Oxygen Species
- Measurement of Respiratory Burst Products Generated by Professional Phagocytes
- The K-562 Cell Model for Analysis of Neutrophil NADPH Oxidase Function
- Cell-Free Assays
- Immunoaffinity Purification of Human Phagocyte Flavocytochrome b and Analysis of Conformational Dynamics
- Analysis of Neutrophil Gene Expression and Transcription Factors
- Genome-Scale Transcript Analyses in Human Neutrophils
- Fast and Accurate Quantitative Analysis of Cytokine Gene Expression in Human Neutrophils by Reverse Transcription Real-Time PCR
- Detection of Intact Transcription Factors in Human Neutrophils
- Neutrophil Defects and Diagnosis
- Disorders of Neutrophil Function
- Diagnostic Assays for Chronic Granulomatous Disease and Other Neutrophil Disorders
- Diagnostic Assays for Myeloperoxidase Deficiency.