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03689nam a22003375i 4500 |
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978-1-59745-526-8 |
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20191025191007.0 |
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cr nn 008mamaa |
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100301s2009 xxu| s |||| 0|eng d |
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|a 9781597455268
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|a 10.1007/978-1-59745-526-8
|2 doi
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|a Sistema de Bibliotecas del Tecnológico de Costa Rica
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| 245 |
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|a Potassium Channels
|b Methods and Protocols /
|c edited by Jonathan D. Lippiat.
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| 250 |
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|a 1st ed. 2009.
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| 260 |
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|a Totowa, NJ :
|b Humana Press :
|b Imprint: Humana,
|c 2009.
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| 300 |
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|a X, 302 p. 59 illus.
|b online resource.
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| 336 |
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|a text
|b txt
|2 rdacontent
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|a computer
|b c
|2 rdamedia
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| 338 |
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|a online resource
|b cr
|2 rdacarrier
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| 490 |
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|a Methods in Molecular Biology,
|v 491
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|a Methods For Studying Potassium Channel Transcription And Mrna Splicing -- Identifying Transcriptional Regulatory Regions Using Reporter Genes and DNA-Protein Interactions by Chromatin Immunoprecipitation -- Quantitative RT-PCR Methods for Investigation of Low Copy Potassium Channel Gene Expression in Native Murine Arteries -- Cloning of Potassium Channel Splice Variants from Tissues and Cells -- Methods for Studying the Cell Biology of Potassium Channel Proteins -- Chemiluminescence Assays to Investigate Membrane Expression and Clathrin-Mediated Endocytosis of KATP Channels -- Investigation of KATP Channel Endocytosis by Immunofluorescence -- Investigation of KATP Channel Endocytosis and Cell Surface Density by Biotinylation and Western Blotting -- Lipid Microdomains and K+ Channel Compartmentation: Detergent and Non-Detergent-Based Methods for the Isolation and Characterisation of Cholesterol-Enriched Lipid Rafts -- Determination of Phosphoinositide Binding to K+ Channel Subunits Using a Protein-Lipid Overlay Assay -- Protein Complex Analysis of Native Brain Potassium Channels by Proteomics -- Electrophysiological Techniques For The Study Of Potassium Channel Function -- Xenopus Oocytes as a Heterologous Expression System for Studying Ion Channels with the Patch-Clamp Technique -- Whole-Cell Recording Using the Perforated Patch Clamp Technique -- Recording the Activity of ATP-Sensitive K+ Channels in Open-Cell Cell-Attached Configuration -- Planar Patch Clamp: Advances in Electrophysiology -- Analysing Steroid Modulation of BKCa Channels Reconstituted into Planar Lipid Bilayers -- Optical Techniques For The Study Of Potassium Channel Function And Interactions -- Using Bioluminescence Resonance Energy Transfer to Measure Ion Channel Assembly -- The Use of FRET Microscopy to Elucidate Steady State Channel Conformational Rearrangements and G Protein Interaction with the GIRK Channels -- The Voltage-Clamp Fluorometry Technique -- Methods For Studying Potassium Channelopathies And Pharmacological Modulators -- Identification of Mutations in the Kir6.2 Subunit of the KATP Channel -- Modulation of Potassium Ion Channel Proteins Utilising Antibodies -- Fluorescence-Based Tl+?Influx Assays as a Novel Approach for Characterization of Small-Conductance Ca2+?Activated K+ Channel Modulators -- Rubidium Efflux as a Tool for the Pharmacological Characterisation of Compounds with BK Channel Opening Properties -- Recording hERG Potassium Currents and Assessing the Effects of Compounds Using the Whole-Cell Patch-Clamp Technique.
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| 650 |
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|a Pharmacology.
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| 650 |
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|a Cell biology.
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| 650 |
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|a Cell membranes .
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| 650 |
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4 |
|a Pharmacology/Toxicology.
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| 650 |
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|a Cell Biology.
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| 650 |
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|a Membrane Biology.
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| 700 |
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|a Lippiat, Jonathan D.
|e editor.
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| 710 |
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|a SpringerLink (Online service)
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| 773 |
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|t Springer eBooks
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| 856 |
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|u https://doi.org/10.1007/978-1-59745-526-8
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