Potassium Channels Methods and Protocols /

Detalles Bibliográficos
Autor Corporativo: SpringerLink (Online service)
Otros Autores: Lippiat, Jonathan D. (Editor )
Formato: eBook
Lenguaje:English
Publicado: Totowa, NJ : Humana Press : Imprint: Humana, 2009.
Edición:1st ed. 2009.
Colección:Methods in Molecular Biology, 491
Materias:
Acceso en línea:https://doi.org/10.1007/978-1-59745-526-8
Tabla de Contenidos:
  • Methods For Studying Potassium Channel Transcription And Mrna Splicing
  • Identifying Transcriptional Regulatory Regions Using Reporter Genes and DNA-Protein Interactions by Chromatin Immunoprecipitation
  • Quantitative RT-PCR Methods for Investigation of Low Copy Potassium Channel Gene Expression in Native Murine Arteries
  • Cloning of Potassium Channel Splice Variants from Tissues and Cells
  • Methods for Studying the Cell Biology of Potassium Channel Proteins
  • Chemiluminescence Assays to Investigate Membrane Expression and Clathrin-Mediated Endocytosis of KATP Channels
  • Investigation of KATP Channel Endocytosis by Immunofluorescence
  • Investigation of KATP Channel Endocytosis and Cell Surface Density by Biotinylation and Western Blotting
  • Lipid Microdomains and K+ Channel Compartmentation: Detergent and Non-Detergent-Based Methods for the Isolation and Characterisation of Cholesterol-Enriched Lipid Rafts
  • Determination of Phosphoinositide Binding to K+ Channel Subunits Using a Protein-Lipid Overlay Assay
  • Protein Complex Analysis of Native Brain Potassium Channels by Proteomics
  • Electrophysiological Techniques For The Study Of Potassium Channel Function
  • Xenopus Oocytes as a Heterologous Expression System for Studying Ion Channels with the Patch-Clamp Technique
  • Whole-Cell Recording Using the Perforated Patch Clamp Technique
  • Recording the Activity of ATP-Sensitive K+ Channels in Open-Cell Cell-Attached Configuration
  • Planar Patch Clamp: Advances in Electrophysiology
  • Analysing Steroid Modulation of BKCa Channels Reconstituted into Planar Lipid Bilayers
  • Optical Techniques For The Study Of Potassium Channel Function And Interactions
  • Using Bioluminescence Resonance Energy Transfer to Measure Ion Channel Assembly
  • The Use of FRET Microscopy to Elucidate Steady State Channel Conformational Rearrangements and G Protein Interaction with the GIRK Channels
  • The Voltage-Clamp Fluorometry Technique
  • Methods For Studying Potassium Channelopathies And Pharmacological Modulators
  • Identification of Mutations in the Kir6.2 Subunit of the KATP Channel
  • Modulation of Potassium Ion Channel Proteins Utilising Antibodies
  • Fluorescence-Based Tl+?Influx Assays as a Novel Approach for Characterization of Small-Conductance Ca2+?Activated K+ Channel Modulators
  • Rubidium Efflux as a Tool for the Pharmacological Characterisation of Compounds with BK Channel Opening Properties
  • Recording hERG Potassium Currents and Assessing the Effects of Compounds Using the Whole-Cell Patch-Clamp Technique.