Toll-Like Receptors Methods and Protocols /

Detalles Bibliográficos
Autor Corporativo: SpringerLink (Online service)
Otros Autores: McCoy, Claire E. (Editor ), O'Neill, Luke A.J. (Editor )
Formato: eBook
Lenguaje:English
Publicado: Totowa, NJ : Humana Press : Imprint: Humana, 2009.
Edición:1st ed. 2009.
Colección:Methods in Molecular Biology, 517
Materias:
Acceso en línea:https://doi.org/10.1007/978-1-59745-541-1
Tabla de Contenidos:
  • Methods to Detect and Analyze Toll-Like Receptors
  • Expression Analysis of the Toll-Like Receptors in Human Peripheral Blood Mononuclear Cells
  • Ligands, Cell-Based Models, and Readouts Required for Toll-Like Receptor Action
  • Toll-Like Receptor Interactions Imaged by FRET Microscopy and GFP Fragment Reconstitution
  • Predicting Toll-Like Receptor Structures and Characterizing Ligand Binding
  • Bioinformatic Analysis of Toll-Like Receptor Sequences and Structures
  • Expression, Purification, and Crystallization of Toll/Interleukin-1 Receptor (TIR) Domains
  • Methods to Analyze Signal Transduction Downstream of Toll-Like Receptor Stimulation
  • Proteomic Analysis of Protein Complexes in Toll-Like Receptor Biology
  • 2D-DIGE: Comparative Proteomics of Cellular Signalling Pathways
  • MAPPIT (Mammalian Protein–Protein Interaction Trap) Analysis of Early Steps in Toll-Like Receptor Signalling
  • Analysis of the Functional Role of Toll-Like Receptor-4 Tyrosine Phosphorylation
  • Analysis of Ubiquitin Degradation and Phosphorylation of Proteins
  • The Generation of Highly Purified Primary Human Neutrophils and Assessment of Apoptosis in Response to Toll-Like Receptor Ligands
  • Cellular Expression of A20 and ABIN-3 in Response to Toll-Like Receptor-4 Stimulation
  • Characterisation of Viral Proteins that Inhibit Toll-Like Receptor Signal Transduction
  • Genetic Techniques in Toll-Like Receptor Analysis
  • Genetic Dissection of Toll-Like Receptor Signaling Using ENU Mutagenesis
  • Microarray Experiments to Uncover Toll-Like Receptor Function
  • Uncovering Novel Gene Function in Toll-Like Receptor Signalling Using siRNA
  • Genotyping Methods to Analyse Polymorphisms in Toll-Like Receptors and Disease
  • Toll-Like Receptors and Disease
  • Experimental Models of Acute Infection and Toll-Like Receptor Driven Septic Shock
  • Toll-Like Receptors and Rheumatoid Arthritis
  • Practical Techniques for Detection of Toll-Like Receptor-4 in the Human Intestine
  • Toll-Like Receptor-Dependent Immune Complex Activation of B Cells and Dendritic Cells
  • Innate Immunity, Toll-Like Receptors, and Atherosclerosis: Mouse Models and Methods
  • Generation of Parasite Antigens for Use in Toll-Like Receptor Research
  • Biomarkers Measuring the Activity of Toll-Like Receptor Ligands in Clinical Development Programs.