Protein Blotting and Detection Methods and Protocols /
| Corporate Author: | |
|---|---|
| Other Authors: | , |
| Format: | eBook |
| Language: | English |
| Published: |
Totowa, NJ :
Humana Press : Imprint: Humana,
2009.
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| Edition: | 1st ed. 2009. |
| Series: | Methods in Molecular Biology,
536 |
| Subjects: | |
| Online Access: | https://doi.org/10.1007/978-1-59745-542-8 |
Table of Contents:
- Origins of Protein Blotting
- Western Blotting: Remembrance of Things Past
- to Protein Blotting
- Simultaneous Immunoblotting Analysis with Activity Gel Electrophoresis and 2-D Gel Electrophoresis
- Diffusion Blotting for Rapid Production of Multiple Identical Imprints from Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis on a Solid Support
- Tryptic Peptide Purification Using Polyvinylidene Difluoride Membrane for Matrix-Assisted Laser Desorption Ionization Time of Flight Mass Spectrometry
- Affinity Immunoblotting for Analysis of Antibody Clonotype Distribution in a Lupus Patient Developing Anti-Ro 60 Over Time
- Nonelectrophoretic Bidirectional Transfer of a Single SDS-PAGE Gel with Multiple Antigens to Obtain 12 Immunoblots
- Slice Blotting
- Localizing Proteins by Tissue Printing
- Dot-Immunobinding Assay
- Capillary Blotting of Glycosaminoglycans on Nitrocellulose Membranes After Agarose-Gel Electrophoresis Separation
- Quantitative computerized western blotting
- Cationic electrophoresis and eastern blotting
- A miniaturized blotting system for simultaneous detecting of differentautoantibodies
- Transfer and Multiplex Immunoblotting of a Paraffin Embedded Tissue
- Multistrip Western Blotting to Increase Quantitative Data Output
- Western Blotting/Edman Sequencing using PVDF Membrane
- Blotting from PhastGel to Membranes by Ultrasound
- Ultrarapid Electrophoretic Transfer of High and Low Molecular Weight Proteins Using Heat
- Strip Immunoblotting of Multiple Antigenic Peptides to Nitrocellulose Membrane
- Affinity Purification of Autoantibodies from an Antigen Strip Excised from a Nitrocellulose Protein Blot
- Double-Blotting: A Solution to the Problem of Nonspecific Binding of Secondary Antibodies in Immunoblotting Procedures
- Efficient Electroblotting of Very Large Proteins Using a Vertical Agarose Electrophoresis System
- Semi-Dry Protein Transfer and Immunodetection of P-Selectin Using an Antibody to its C-terminal Tag
- The Multiple Antigen Blot Assay: A Simple, Versatile and Multipurpose Immunoenzymatic Technique
- Blotting from Immobilized pH Gradient Gels: Application to Total Cell Lysates
- Immunoprecipitation-Western Blot for Proteins of Low Abundance
- Native Electrophoresis and Western Blot Analysis: Method and Applications
- Grid-Immunoblotting
- Sequential Use of Immunoblots for Characterization of Autoantibody Specificities
- Renaturation of Recombinant Ro 60 Autoantigen by Calcium Ions on PVDF Membrane
- Intermittent Microwave Irradiation Facilitates Antigen-Antibody Reaction in Western Blot Analysis
- Detection of Protein-Protein Interactions by Far-Western Blotting
- On-Membrane Tryptic Digestion of Proteins for Mass Spectrometry Analysis
- Western Blot Analysis of Adhesive Interactions under Fluid Shear Conditions: The Blot Rolling Assay
- ELISPOT Assay on Membrane Microplates
- A Brief Review of Other Notable Protein Blotting Methods
- Phosphoprotein Detection on Protein Electroblot Using a Phosphate-Specific Fluorophore
- Rapid, Antibody-Free Detection of Recombinant Proteins on Blots Using Enzyme Fragment Complementation
- Fluorescent Labeling of Proteins and Its Application to SDS-PAGE and Western Blotting
- Whole and Strip Nitrocellulose Membrane as well as a New-Line-Immunoblotting ofAntigen Using the Chemiluminescence Technique
- Protein Stains to Detect Antigen on Membranes
- Use of non-radioactive detection method for North- and South-Western Blot
- Immunoblotting using Radiolabeled Reagents for Detection
- Detection of Protein Carbonyls by Means of Biotin Hydrazide-Streptavidin Affinity Methods
- "Rainbow" Western Blotting
- Multiple antigen detection (MAD) western blotting
- Detection of calcium binding by Ro 60 multiple antigenic peptides on nitrocellulose membrane using Quin-2-
- Detection of La/SS-B by Western Blot using Nanogold-Tagged Antibodies andSilver Enhancement
- Imaging Systems for Westerns: Chemiluminescence vs. Infrared Detection
- A Fluorescent Codetection System for Immunoblotting and Proteomics Through ECL-Plex and CyDye Labeling
- Visualization of Unstained Protein Bands on PVDF
- Improved Protein Detection Using Cold Microwave Technology
- TLC Blot (Far-Eastern Blot) and Its Applications
- A Brief Review of Other Notable Protein Detection Methods on Blots
- Sending Secret Messages on Nitrocellulose Membrane and the Use of a Molecular Pen for Orientation in ECL Membrane Assays.