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03042nam a22003855i 4500 |
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978-1-59745-566-4 |
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20191027042513.0 |
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cr nn 008mamaa |
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100301s2009 xxu| s |||| 0|eng d |
| 020 |
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|a 9781597455664
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7 |
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|a 10.1007/978-1-59745-566-4
|2 doi
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| 040 |
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|a Sistema de Bibliotecas del Tecnológico de Costa Rica
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| 245 |
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|a SUMO Protocols
|c edited by Helle Ulrich.
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| 250 |
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|a 1st ed. 2009.
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| 260 |
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|a Totowa, NJ :
|b Humana Press :
|b Imprint: Humana,
|c 2009.
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| 300 |
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|a X, 324 p. 70 illus.
|b online resource.
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| 336 |
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|a text
|b txt
|2 rdacontent
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| 337 |
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|a computer
|b c
|2 rdamedia
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| 338 |
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|a online resource
|b cr
|2 rdacarrier
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| 490 |
1 |
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|a Methods in Molecular Biology,
|v 497
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|a The SUMO System: An Overview -- Identification of Sumo Targets -- Identification of SUMO Target Proteins by Quantitative Proteomics -- Identification of SUMO-Conjugated Proteins and their SUMO Attachment Sites Using Proteomic Mass Spectrometry -- Identification of SUMO Targets Through In Vitro Expression Cloning -- Enhanced Detection of In Vivo SUMO Conjugation by Ubc9 Fusion-Dependent Sumoylation (UFDS) -- In Vivo Detection and Characterization of Sumoylation Targets in Saccharomyces cerevisiae -- Identification Of Sumo Interactors -- Identification of SUMO-Interacting Proteins by Yeast Two-Hybrid Analysis -- Identification of SUMO-Binding Motifs by NMR -- Functional Analysis Of Sumoylation In Vivo -- Regulation of Transcription Factor Activity by SUMO Modification -- Characterization of the Effects and Functions of Sumoylation Through Rapamycin-Mediated Heterodimerization -- Biochemical Studies Of Sumo Conjugation -- Purification of SUMO Conjugating Enzymes and Kinetic Analysis of Substrate Conjugation -- Performing In Vitro Sumoylation Reactions Using Recombinant Enzymes -- Preparation of Sumoylated Substrates for Biochemical Analysis -- Strategies for the Expression of SUMO-Modified Target Proteins in Escherichia coli -- Biochemical Studies of Sumo Deconjugation -- Preparation of SUMO Proteases and Kinetic Analysis Using Endogenous Substrates -- An In Vitro FRET-Based Assay for the Analysis of SUMO Conjugation and Isopeptidase Cleavage -- FRET-Based In Vitro Assays for the Analysis of SUMO Protease Activities -- Detection and Characterization of SUMO Protease Activity Using a Sensitive Enzyme-Based Reporter Assay -- Manipulation And Alternative Uses Of The Sumo System -- Inhibition of the SUMO Pathway by Gam1 -- SUMO Fusion Technology for Enhanced Protein Production in Prokaryotic and Eukaryotic Expression Systems.
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| 650 |
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|a Proteins .
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| 650 |
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|a Cell biology.
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| 650 |
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|a Biochemistry.
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| 650 |
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|a Proteomics.
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| 650 |
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|a Enzymology.
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| 650 |
1 |
4 |
|a Protein Science.
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| 650 |
2 |
4 |
|a Cell Biology.
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| 650 |
2 |
4 |
|a Biochemistry, general.
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| 650 |
2 |
4 |
|a Proteomics.
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| 650 |
2 |
4 |
|a Enzymology.
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| 700 |
1 |
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|a Ulrich, Helle.
|e editor.
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| 710 |
2 |
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|a SpringerLink (Online service)
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| 773 |
0 |
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|t Springer eBooks
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| 856 |
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0 |
|u https://doi.org/10.1007/978-1-59745-566-4
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