SUMO Protocols
| Autor Corporativo: | |
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| Otros Autores: | |
| Formato: | eBook |
| Lenguaje: | English |
| Publicado: |
Totowa, NJ :
Humana Press : Imprint: Humana,
2009.
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| Edición: | 1st ed. 2009. |
| Colección: | Methods in Molecular Biology,
497 |
| Materias: | |
| Acceso en línea: | https://doi.org/10.1007/978-1-59745-566-4 |
Tabla de Contenidos:
- The SUMO System: An Overview
- Identification of Sumo Targets
- Identification of SUMO Target Proteins by Quantitative Proteomics
- Identification of SUMO-Conjugated Proteins and their SUMO Attachment Sites Using Proteomic Mass Spectrometry
- Identification of SUMO Targets Through In Vitro Expression Cloning
- Enhanced Detection of In Vivo SUMO Conjugation by Ubc9 Fusion-Dependent Sumoylation (UFDS)
- In Vivo Detection and Characterization of Sumoylation Targets in Saccharomyces cerevisiae
- Identification Of Sumo Interactors
- Identification of SUMO-Interacting Proteins by Yeast Two-Hybrid Analysis
- Identification of SUMO-Binding Motifs by NMR
- Functional Analysis Of Sumoylation In Vivo
- Regulation of Transcription Factor Activity by SUMO Modification
- Characterization of the Effects and Functions of Sumoylation Through Rapamycin-Mediated Heterodimerization
- Biochemical Studies Of Sumo Conjugation
- Purification of SUMO Conjugating Enzymes and Kinetic Analysis of Substrate Conjugation
- Performing In Vitro Sumoylation Reactions Using Recombinant Enzymes
- Preparation of Sumoylated Substrates for Biochemical Analysis
- Strategies for the Expression of SUMO-Modified Target Proteins in Escherichia coli
- Biochemical Studies of Sumo Deconjugation
- Preparation of SUMO Proteases and Kinetic Analysis Using Endogenous Substrates
- An In Vitro FRET-Based Assay for the Analysis of SUMO Conjugation and Isopeptidase Cleavage
- FRET-Based In Vitro Assays for the Analysis of SUMO Protease Activities
- Detection and Characterization of SUMO Protease Activity Using a Sensitive Enzyme-Based Reporter Assay
- Manipulation And Alternative Uses Of The Sumo System
- Inhibition of the SUMO Pathway by Gam1
- SUMO Fusion Technology for Enhanced Protein Production in Prokaryotic and Eukaryotic Expression Systems.