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02434nam a22003615i 4500 |
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978-1-60327-019-9 |
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20190617193944.0 |
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cr nn 008mamaa |
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100301s2009 xxu| s |||| 0|eng d |
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|a 9781603270199
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7 |
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|a 10.1007/978-1-60327-019-9
|2 doi
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| 040 |
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|a Sistema de Bibliotecas del Tecnológico de Costa Rica
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| 245 |
1 |
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|a Transgenesis Techniques
|b Principles and Protocols /
|c edited by Elizabeth J. Cartwright.
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| 250 |
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|a 3rd ed. 2009.
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| 260 |
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|a Totowa, NJ :
|b Humana Press :
|b Imprint: Humana,
|c 2009.
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| 300 |
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|a XII, 335 p.
|b online resource.
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| 336 |
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|a text
|b txt
|2 rdacontent
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| 337 |
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|a computer
|b c
|2 rdamedia
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| 338 |
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|a online resource
|b cr
|2 rdacarrier
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| 490 |
1 |
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|a Methods in Molecular Biology,
|v 561
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| 505 |
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|a Transgenesis in Various Model Systems -- Transgenesis in Drosophila melanogaster -- Transgenesis in Caenorhabditis elegans -- Transgenesis in Zebrafish with the Tol2 Transposon System -- Generation of Transgenic Frogs -- Pronuclear DNA Injection for the Production of Transgenic Rats -- Transgenesis in the Mouse -- Cell-Type-Specific Transgenesis in the Mouse -- Transgene Design and Delivery into the Mouse Genome: Keys to Success -- Overexpression Transgenesis in Mouse: Pronuclear Injection -- Gene-Targeting Vectors -- Gene Trap: Knockout on the Fast Lane -- Culture of Murine Embryonic Stem Cells -- Targeting Embryonic Stem Cells -- Generation of Chimeras by Microinjection -- Generation of Chimeras by Morula Aggregation -- Surgical Techniques for the Generation of Mutant Mice -- Site-Specific Recombinases for Manipulation of the Mouse Genome -- Cre Transgenic Mouse Lines -- Large-Scale Mouse Mutagenesis -- Dedicated Mouse Production and Husbandry -- Biological Methods for Archiving and Maintaining Mutant Laboratory Mice. Part I: Conserving Mutant Strains -- Biological Methods for Archiving and Maintaining Mutant Laboratory Mice. Part II: Recovery and Distribution of Conserved Mutant Strains.
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| 650 |
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|a Genetic engineering.
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| 650 |
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|a Human genetics.
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| 650 |
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|a Animal genetics.
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| 650 |
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|a Transgenic organisms.
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| 650 |
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4 |
|a Genetic Engineering.
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| 650 |
2 |
4 |
|a Human Genetics.
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| 650 |
2 |
4 |
|a Animal Genetics and Genomics.
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| 650 |
2 |
4 |
|a Transgenics.
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| 700 |
1 |
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|a Cartwright, Elizabeth J.
|e editor.
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| 710 |
2 |
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|a SpringerLink (Online service)
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| 773 |
0 |
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|t Springer eBooks
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| 856 |
4 |
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|u https://doi.org/10.1007/978-1-60327-019-9
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