Mesenchymal Stem Cells Methods and Protocols /

Bibliographic Details
Corporate Author: SpringerLink (Online service)
Other Authors: Prockop, Darwin J. (Editor), Phinney, Donald G. (Editor), Bunnell, Bruce A. (Editor)
Format: eBook
Language:English
Published: Totowa, NJ : Humana Press : Imprint: Humana, 2008.
Edition:1st ed. 2008.
Series:Methods in Molecular Biology, 449
Subjects:
Online Access:https://doi.org/10.1007/978-1-60327-169-1
Table of Contents:
  • Isolation of Human Multipotential Stromal Cells from Bone Marrow and Adipose Tissue
  • Isolation and Culture of Bone Marrow-Derived Human Multipotent Stromal Cells (hMSCs)
  • Mesenchymal Stem Cells from Adult Bone Marrow
  • A Method to Isolate and Purify Human Bone Marrow Stromal Stem Cells
  • Adipose-Derived Stem Cells
  • Isolation of Human Adipose-derived Stem Cells from Biopsies and Liposuction Specimens
  • Manipulation of Human Multipotential Stromal Cells
  • Colony Forming Unit Assays for MSCs
  • Differentiation and Characterization of Human MSCs
  • Freezing Harvested hMSCs and Recovery of hMSCs from Frozen Vials for Subsequent Expansion, Analysis, and Experimentation
  • Gene Expression Analysis at the Single Cell Level Using the Human Bone Marrow Stromal Cell as a Model: Sample Preparation Methods
  • Assays of MSCs with Microarrays
  • Gene Delivery to Mesenchymal Stem Cells
  • Isolation and Characterization of Murine Multipotential Stromal Cells from Bone Marrow
  • Isolation of Mesenchymal Stem Cells from Murine Bone Marrow by Immunodepletion.