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03429nam a22003495i 4500 |
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978-1-60327-310-7 |
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20191027193222.0 |
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cr nn 008mamaa |
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100301s2009 xxu| s |||| 0|eng d |
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|a 9781603273107
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024 |
7 |
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|a 10.1007/978-1-60327-310-7
|2 doi
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|a Sistema de Bibliotecas del Tecnológico de Costa Rica
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245 |
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|a Membrane Proteomics
|b Methods and Protocols /
|c edited by Matthew J. Peirce, Robin Wait.
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250 |
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|a 1st ed. 2009.
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|a Totowa, NJ :
|b Humana Press :
|b Imprint: Humana,
|c 2009.
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|a XIII, 342 p.
|b online resource.
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|a text
|b txt
|2 rdacontent
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|a computer
|b c
|2 rdamedia
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|a online resource
|b cr
|2 rdacarrier
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|a Methods in Molecular Biology,
|v 528
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|a In Silico Methods for Prediction of Membrane Protein Hydrophobicity and Topology -- Online Tools for Predicting Integral Membrane Proteins -- In Silico Identification of Novel G Protein Coupled Receptors -- Transcriptome-Based Identification of Candidate Membrane Proteins -- Extraction and Purification of Membrane Proteins -- Separation of Thylakoid Membrane Proteins by Sucrose Gradient Ultracentrifugation or Blue Native-SDS-PAGE Two-Dimensional Electrophoresis -- Extraction of Yeast Mitochondrial Membrane Proteins by Solubilization and Detergent/Polymer Aqueous Two-Phase Partitioning -- 16-BAC/SDS-PAGE Analysis of Membrane Proteins of Yeast Mitochondria Purified by Free Flow Electrophoresis -- Sequential Detergent Extraction Prior to Mass Spectrometry Analysis -- Enrichment of Brain Plasma Membranes by Affinity Two-Phase Partitioning -- Protocol to Enrich and Analyze Plasma Membrane Proteins -- Proteomic Analysis of the Lymphocyte Plasma Membrane Using Cell Surface Biotinylation and Solution-Phase Isoelectric Focusing -- Identification of Target Membrane Proteins as Detected by Phage Antibodies -- Membrane Protease Degradomics: Proteomic Identification and Quantification of Cell Surface Protease Substrates -- Purification of Basolateral Integral Membrane Proteins by Cationic Colloidal Silica-Based Apical Membrane Subtraction -- Moving Closer to the Lipid Raft Proteome Using Quantitative Proteomics -- Use of Sequential Chemical Extractions to Purify Nuclear Membrane Proteins for Proteomics Identification -- Isolation of Extracellular Membranous Vesicles for Proteomic Analysis -- Separation of Membrane Proteins -- Enrichment of Human Platelet Membranes for Proteomic Analysis -- Detergents and Chaotropes for Protein Solubilization Before Two-Dimensional Electrophoresis -- Two-Dimensional Separation of Membrane Proteins by 16-BAC-SDS-PAGE -- Identification and Quantification of Membrane Proteins -- MudPIT Analysis: Application to Human Heart Tissue -- Liquid Chromatography MALDI MS/MS for Membrane Proteome Analysis -- Cysteinyl-Tagging of Integral Membrane Proteins for Proteomic Analysis Using Liquid Chromatography-Tandem Mass Spectrometry -- Quantitative Proteomics Analysis of Pancreatic Zymogen Granule Membrane Proteins.
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650 |
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|a Biochemistry.
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650 |
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|a Proteins .
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650 |
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|a Cell membranes .
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650 |
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4 |
|a Biochemistry, general.
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650 |
2 |
4 |
|a Protein Science.
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650 |
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4 |
|a Membrane Biology.
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700 |
1 |
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|a Peirce, Matthew J.
|e editor.
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700 |
1 |
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|a Wait, Robin.
|e editor.
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710 |
2 |
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|a SpringerLink (Online service)
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773 |
0 |
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|t Springer eBooks
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856 |
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|u https://doi.org/10.1007/978-1-60327-310-7
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