Membrane Proteomics Methods and Protocols /

Detalles Bibliográficos
Autor Corporativo: SpringerLink (Online service)
Otros Autores: Peirce, Matthew J. (Editor ), Wait, Robin. (Editor )
Formato: eBook
Lenguaje:English
Publicado: Totowa, NJ : Humana Press : Imprint: Humana, 2009.
Edición:1st ed. 2009.
Colección:Methods in Molecular Biology, 528
Materias:
Acceso en línea:https://doi.org/10.1007/978-1-60327-310-7
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040 |a Sistema de Bibliotecas del Tecnológico de Costa Rica 
245 1 0 |a Membrane Proteomics  |b Methods and Protocols /  |c edited by Matthew J. Peirce, Robin Wait. 
250 |a 1st ed. 2009. 
260 # # |a Totowa, NJ :  |b Humana Press :  |b Imprint: Humana,  |c 2009. 
300 |a XIII, 342 p.  |b online resource. 
336 |a text  |b txt  |2 rdacontent 
337 |a computer  |b c  |2 rdamedia 
338 |a online resource  |b cr  |2 rdacarrier 
490 1 |a Methods in Molecular Biology,  |v 528 
505 0 |a In Silico Methods for Prediction of Membrane Protein Hydrophobicity and Topology -- Online Tools for Predicting Integral Membrane Proteins -- In Silico Identification of Novel G Protein Coupled Receptors -- Transcriptome-Based Identification of Candidate Membrane Proteins -- Extraction and Purification of Membrane Proteins -- Separation of Thylakoid Membrane Proteins by Sucrose Gradient Ultracentrifugation or Blue Native-SDS-PAGE Two-Dimensional Electrophoresis -- Extraction of Yeast Mitochondrial Membrane Proteins by Solubilization and Detergent/Polymer Aqueous Two-Phase Partitioning -- 16-BAC/SDS-PAGE Analysis of Membrane Proteins of Yeast Mitochondria Purified by Free Flow Electrophoresis -- Sequential Detergent Extraction Prior to Mass Spectrometry Analysis -- Enrichment of Brain Plasma Membranes by Affinity Two-Phase Partitioning -- Protocol to Enrich and Analyze Plasma Membrane Proteins -- Proteomic Analysis of the Lymphocyte Plasma Membrane Using Cell Surface Biotinylation and Solution-Phase Isoelectric Focusing -- Identification of Target Membrane Proteins as Detected by Phage Antibodies -- Membrane Protease Degradomics: Proteomic Identification and Quantification of Cell Surface Protease Substrates -- Purification of Basolateral Integral Membrane Proteins by Cationic Colloidal Silica-Based Apical Membrane Subtraction -- Moving Closer to the Lipid Raft Proteome Using Quantitative Proteomics -- Use of Sequential Chemical Extractions to Purify Nuclear Membrane Proteins for Proteomics Identification -- Isolation of Extracellular Membranous Vesicles for Proteomic Analysis -- Separation of Membrane Proteins -- Enrichment of Human Platelet Membranes for Proteomic Analysis -- Detergents and Chaotropes for Protein Solubilization Before Two-Dimensional Electrophoresis -- Two-Dimensional Separation of Membrane Proteins by 16-BAC-SDS-PAGE -- Identification and Quantification of Membrane Proteins -- MudPIT Analysis: Application to Human Heart Tissue -- Liquid Chromatography MALDI MS/MS for Membrane Proteome Analysis -- Cysteinyl-Tagging of Integral Membrane Proteins for Proteomic Analysis Using Liquid Chromatography-Tandem Mass Spectrometry -- Quantitative Proteomics Analysis of Pancreatic Zymogen Granule Membrane Proteins. 
650 0 |a Biochemistry. 
650 0 |a Proteins . 
650 0 |a Cell membranes . 
650 1 4 |a Biochemistry, general. 
650 2 4 |a Protein Science. 
650 2 4 |a Membrane Biology. 
700 1 |a Peirce, Matthew J.  |e editor. 
700 1 |a Wait, Robin.  |e editor. 
710 2 |a SpringerLink (Online service) 
773 0 |t Springer eBooks 
856 4 0 |u https://doi.org/10.1007/978-1-60327-310-7