Membrane Proteomics Methods and Protocols /

Detalles Bibliográficos
Autor Corporativo: SpringerLink (Online service)
Otros Autores: Peirce, Matthew J. (Editor ), Wait, Robin. (Editor )
Formato: eBook
Lenguaje:English
Publicado: Totowa, NJ : Humana Press : Imprint: Humana, 2009.
Edición:1st ed. 2009.
Colección:Methods in Molecular Biology, 528
Materias:
Acceso en línea:https://doi.org/10.1007/978-1-60327-310-7
Tabla de Contenidos:
  • In Silico Methods for Prediction of Membrane Protein Hydrophobicity and Topology
  • Online Tools for Predicting Integral Membrane Proteins
  • In Silico Identification of Novel G Protein Coupled Receptors
  • Transcriptome-Based Identification of Candidate Membrane Proteins
  • Extraction and Purification of Membrane Proteins
  • Separation of Thylakoid Membrane Proteins by Sucrose Gradient Ultracentrifugation or Blue Native-SDS-PAGE Two-Dimensional Electrophoresis
  • Extraction of Yeast Mitochondrial Membrane Proteins by Solubilization and Detergent/Polymer Aqueous Two-Phase Partitioning
  • 16-BAC/SDS-PAGE Analysis of Membrane Proteins of Yeast Mitochondria Purified by Free Flow Electrophoresis
  • Sequential Detergent Extraction Prior to Mass Spectrometry Analysis
  • Enrichment of Brain Plasma Membranes by Affinity Two-Phase Partitioning
  • Protocol to Enrich and Analyze Plasma Membrane Proteins
  • Proteomic Analysis of the Lymphocyte Plasma Membrane Using Cell Surface Biotinylation and Solution-Phase Isoelectric Focusing
  • Identification of Target Membrane Proteins as Detected by Phage Antibodies
  • Membrane Protease Degradomics: Proteomic Identification and Quantification of Cell Surface Protease Substrates
  • Purification of Basolateral Integral Membrane Proteins by Cationic Colloidal Silica-Based Apical Membrane Subtraction
  • Moving Closer to the Lipid Raft Proteome Using Quantitative Proteomics
  • Use of Sequential Chemical Extractions to Purify Nuclear Membrane Proteins for Proteomics Identification
  • Isolation of Extracellular Membranous Vesicles for Proteomic Analysis
  • Separation of Membrane Proteins
  • Enrichment of Human Platelet Membranes for Proteomic Analysis
  • Detergents and Chaotropes for Protein Solubilization Before Two-Dimensional Electrophoresis
  • Two-Dimensional Separation of Membrane Proteins by 16-BAC-SDS-PAGE
  • Identification and Quantification of Membrane Proteins
  • MudPIT Analysis: Application to Human Heart Tissue
  • Liquid Chromatography MALDI MS/MS for Membrane Proteome Analysis
  • Cysteinyl-Tagging of Integral Membrane Proteins for Proteomic Analysis Using Liquid Chromatography-Tandem Mass Spectrometry
  • Quantitative Proteomics Analysis of Pancreatic Zymogen Granule Membrane Proteins.