Experiments in Molecular Biology
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| Format: | eBook |
| Language: | English |
| Published: |
Totowa, NJ :
Humana Press : Imprint: Humana,
1986.
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| Edition: | 1st ed. 1986. |
| Subjects: | |
| Online Access: | https://doi.org/10.1007/978-1-60327-405-0 |
Table of Contents:
- DNA Isolation and Transformation in Escherichia coli
- Isolation of Plasmid DNA
- Restriction Endonuclease Digestion and Agarose Gel Electrophoresis of DNA
- Restriction Site Mapping
- Southern Blotting
- Preparative Electrophoresis
- DNA Ligation and Escherichia coli Transformation
- Detection of Plasmid by Colony Hybridization
- Microisolation and Microanalysis of Plasmid DNA
- Isopycnic Centrifugation of DNA
- Purification of Plant Ribosomal DNA
- The Extraction and Affinity Chromatography of Messenger RNA
- Agarose Gel Electrophoresis of RNA
- In Vitro Protein Synthesis
- SDS Polyacrylamide Gel Electrophoresis of Proteins
- Isoelectric Focusing (IEF) of Proteins in Thin-Layer Polyacrylamide Gels
- Protein (Western) Blotting with Immunodetection
- Thin-Layer Gel Filtration
- The Dansyl-Edman Method for Peptide Sequencing
- The Double Immunodiffusion Technique
- Immunoelectrophoretic Techniques
- The Time Course of ?-Galactosidase Induction in Escherichia coli
- Experiments on Enzyme Induction in the Unicellular Green Alga Chlorella fusca
- Yeast Protoplast Fusion
- The Ames Test.