The Nucleus Volume 2: Chromatin, Transcription, Envelope, Proteins, Dynamics, and Imaging /
| Autor Corporativo: | |
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| Otros Autores: | |
| Formato: | eBook |
| Lenguaje: | English |
| Publicado: |
Totowa, NJ :
Humana Press : Imprint: Humana,
2008.
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| Edición: | 1st ed. 2008. |
| Colección: | Methods in Molecular Biology,
464 |
| Materias: | |
| Acceso en línea: | https://doi.org/10.1007/978-1-60327-461-6 |
Tabla de Contenidos:
- Physical and Mechanical Properties
- Physical Properties of the Nucleus Studied by Micropipette Aspiration
- Mechanical Properties of Interphase Nuclei Probed by Cellular Strain Application
- Chromatin and Transcription
- Gene Expression in Polytene Nuclei
- Electron Microscope Visualization of RNA Transcription and Processing in Saccharomyces cerevisiae by Miller Chromatin Spreading
- Combing Genomic DNA for Structural and Functional Studies
- Using Molecular Beacons to Study Dispersal of mRNPs from the Gene Locus
- Mapping Cis- and Trans- Chromatin Interaction Networks Using Chromosome Conformation Capture (3C)
- Recognition Imaging of Chromatin and Chromatin-Remodeling Complexes in the Atomic Force Microscope
- Using Cells Encapsulated in Agarose Microbeads to Analyse Nuclear Structure and Functions
- The Nuclear Envelope
- Investigation of Nuclear Envelope Structure and Passive Permeability
- Reconstitution of Nuclear Import in Permeabilized Cells
- Nuclear Envelope Formation In Vitro: A Sea Urchin Egg Cell-Free System
- Modifications of Nuclear Proteins
- Detection and Analysis of (O-linked ?-N-Acetylglucosamine)-Modified Proteins
- Detection of Sumoylated Proteins
- Detection of the Nuclear Poly(ADP-ribose)-Metabolizing Enzymes and Activities in Response to DNA Damage
- Purification and Analysis of Variant and Modified Histones Using 2D PAGE
- Quantification of Redox Conditions in the Nucleus
- Protein Dynamics in the Nucleus
- Fluorescence Correlation Spectroscopy to Assess the Mobility of Nuclear Proteins
- Single Molecule Tracking for Studying Nucleocytoplasmic Transport and Intranuclear Dynamics
- Fluorescence Recovery After Photobleaching (FRAP) to Study Nuclear Protein Dynamics in Living Cells
- Imaging Methods
- Nanosizing by Spatially Modulated Illumination (SMI) Microscopy and Applications to the Nucleus
- Visualisation of RNA by Electron Microscopic In Situ Hybridisation
- Electron Spectroscopic Imaging of the Nuclear Landscape
- Cryoelectron Microscopy of Vitreous Sections: A Step Further Towards the Native State.