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04012nam a22003495i 4500 |
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978-1-60327-483-8 |
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20191027061623.0 |
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cr nn 008mamaa |
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100301s2009 xxu| s |||| 0|eng d |
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|a 9781603274838
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|a 10.1007/978-1-60327-483-8
|2 doi
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| 040 |
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|a Sistema de Bibliotecas del Tecnológico de Costa Rica
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| 245 |
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|a Molecular Embryology
|b Methods and Protocols /
|c edited by Paul Sharpe, Ivor Mason.
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| 250 |
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|a 2nd ed. 2009.
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| 260 |
# |
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|a Totowa, NJ :
|b Humana Press :
|b Imprint: Humana,
|c 2009.
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| 300 |
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|a XVII, 798 p.
|b online resource.
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| 336 |
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|a text
|b txt
|2 rdacontent
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| 337 |
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|a computer
|b c
|2 rdamedia
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| 338 |
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|a online resource
|b cr
|2 rdacarrier
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| 490 |
1 |
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|a Methods in Molecular Biology,
|v 461
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| 505 |
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|a The Mouse Embryo -- The Mouse as a Developmental Model -- Culture of Postimplantation Mouse Embryos -- Organ Culture in the Analysis of Tissue Interactions -- Treatment of Mice with Retinoids In Vivo and In Vitro -- Analysis of Skeletal Ontogenesis through Differential Staining of Bone and Cartilage -- Cell Grafting and Labeling in Postimplantation Mouse Embryos -- Production of Transgenic Rodents by the Microinjection of Cloned DNA into Fertilized One-Celled Eggs -- Cre Recombinase Mediated Alterations of the Mouse Genome Using Embryonic Stem Cells -- Gene Trapping in Mouse Embryonic Stem Cells -- Application of lacZ Transgenic Mice to Cell Lineage Studies -- Transgenic RNA Interference to Investigate Gene Function in the Mouse -- Mouse Primordial Germ Cells -- Gene Transfer to the Rodent Embryo by Retroviral Vectors -- The Chicken Embryo -- The Avian Embryo -- Chick Embryos -- New Culture -- EC Culture -- Grafting Hensen's Node -- Grafting of Somites -- Microsurgical Manipulation of the Notochord -- Transplantation of Avian Neural Tissue -- Grafting of Apical Ridge and Polarizing Region -- Tissue Recombinations in Collagen Gels -- Quail—Chick Chimeras -- Using Fluorescent Dyes for Fate Mapping, Lineage Analysis, and Axon Tracing in the Chick Embryo -- Gene Transfer in Avian Embryos Using Replication-Competent Retroviruses -- Electroporation in Avian Embryos -- Amphibian Embryos -- An Overview of Xenopus Development -- Mesoderm Induction Assays -- Experimental Embryological Methods for Analysis of Neural Induction in the Amphibian -- A Method for Generating Transgenic Frog Embryos -- Axolotl/Newt -- Zebrafish And Medaka -- The Zebrafish -- Small-Scale Marker-Based Screening for Mutations in Zebrafish Development -- Microinjection and Cell Transplantation in Zebrafish Embryos -- Recent Advances in Meganuclease-and Transposon-Mediated Transgenesis of Medaka and Zebrafish -- Retinoids in Nonmammalian Embryos -- Nonvertebrate Chordates -- Protochordates -- Molecular Techniques -- Subtractive Hybridization and Construction of cDNA Libraries -- Differential Display of Eukaryotic mRNA -- Using DNA Microarrays -- Profiling Gene Transcription in the Developing Embryo -- RT-PCR on Embryos Using Degenerate Oligonucleotide Primers -- Single-Cell RT-PCR cDNA Subtraction -- In-Situ Hybridization of Radioactive Riboprobes to RNA in Tissue Sections -- In-Situ Hybridization and Immunohistochemistry in Whole Embryos -- Wholemount In Situ Hybridization to Xenopus Embryos -- Wholemount In Situ Hybridization to Amphioxus Embryos -- In-Situ Hybridization to Sections (Nonradioactive) -- Immunohistochemistry Using Polyester Wax -- Immunohistochemistry on Whole Embryos -- Whole Embryo Assays for Programmed Cell Death -- Protein Techniques -- Microscopy and Photography -- Microscopy and Photomicrography Techniques.
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| 650 |
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0 |
|a Biochemistry.
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| 650 |
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0 |
|a Cell biology.
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| 650 |
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0 |
|a Developmental biology.
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| 650 |
1 |
4 |
|a Biochemistry, general.
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| 650 |
2 |
4 |
|a Cell Biology.
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| 650 |
2 |
4 |
|a Developmental Biology.
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| 700 |
1 |
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|a Sharpe, Paul.
|e editor.
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| 700 |
1 |
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|a Mason, Ivor.
|e editor.
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| 710 |
2 |
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|a SpringerLink (Online service)
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| 773 |
0 |
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|t Springer eBooks
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| 856 |
4 |
0 |
|u https://doi.org/10.1007/978-1-60327-483-8
|