Molecular Embryology Methods and Protocols /

Detalles Bibliográficos
Autor Corporativo: SpringerLink (Online service)
Otros Autores: Sharpe, Paul. (Editor ), Mason, Ivor. (Editor )
Formato: eBook
Lenguaje:English
Publicado: Totowa, NJ : Humana Press : Imprint: Humana, 2009.
Edición:2nd ed. 2009.
Colección:Methods in Molecular Biology, 461
Materias:
Acceso en línea:https://doi.org/10.1007/978-1-60327-483-8
Tabla de Contenidos:
  • The Mouse Embryo
  • The Mouse as a Developmental Model
  • Culture of Postimplantation Mouse Embryos
  • Organ Culture in the Analysis of Tissue Interactions
  • Treatment of Mice with Retinoids In Vivo and In Vitro
  • Analysis of Skeletal Ontogenesis through Differential Staining of Bone and Cartilage
  • Cell Grafting and Labeling in Postimplantation Mouse Embryos
  • Production of Transgenic Rodents by the Microinjection of Cloned DNA into Fertilized One-Celled Eggs
  • Cre Recombinase Mediated Alterations of the Mouse Genome Using Embryonic Stem Cells
  • Gene Trapping in Mouse Embryonic Stem Cells
  • Application of lacZ Transgenic Mice to Cell Lineage Studies
  • Transgenic RNA Interference to Investigate Gene Function in the Mouse
  • Mouse Primordial Germ Cells
  • Gene Transfer to the Rodent Embryo by Retroviral Vectors
  • The Chicken Embryo
  • The Avian Embryo
  • Chick Embryos
  • New Culture
  • EC Culture
  • Grafting Hensen's Node
  • Grafting of Somites
  • Microsurgical Manipulation of the Notochord
  • Transplantation of Avian Neural Tissue
  • Grafting of Apical Ridge and Polarizing Region
  • Tissue Recombinations in Collagen Gels
  • Quail—Chick Chimeras
  • Using Fluorescent Dyes for Fate Mapping, Lineage Analysis, and Axon Tracing in the Chick Embryo
  • Gene Transfer in Avian Embryos Using Replication-Competent Retroviruses
  • Electroporation in Avian Embryos
  • Amphibian Embryos
  • An Overview of Xenopus Development
  • Mesoderm Induction Assays
  • Experimental Embryological Methods for Analysis of Neural Induction in the Amphibian
  • A Method for Generating Transgenic Frog Embryos
  • Axolotl/Newt
  • Zebrafish And Medaka
  • The Zebrafish
  • Small-Scale Marker-Based Screening for Mutations in Zebrafish Development
  • Microinjection and Cell Transplantation in Zebrafish Embryos
  • Recent Advances in Meganuclease-and Transposon-Mediated Transgenesis of Medaka and Zebrafish
  • Retinoids in Nonmammalian Embryos
  • Nonvertebrate Chordates
  • Protochordates
  • Molecular Techniques
  • Subtractive Hybridization and Construction of cDNA Libraries
  • Differential Display of Eukaryotic mRNA
  • Using DNA Microarrays
  • Profiling Gene Transcription in the Developing Embryo
  • RT-PCR on Embryos Using Degenerate Oligonucleotide Primers
  • Single-Cell RT-PCR cDNA Subtraction
  • In-Situ Hybridization of Radioactive Riboprobes to RNA in Tissue Sections
  • In-Situ Hybridization and Immunohistochemistry in Whole Embryos
  • Wholemount In Situ Hybridization to Xenopus Embryos
  • Wholemount In Situ Hybridization to Amphioxus Embryos
  • In-Situ Hybridization to Sections (Nonradioactive)
  • Immunohistochemistry Using Polyester Wax
  • Immunohistochemistry on Whole Embryos
  • Whole Embryo Assays for Programmed Cell Death
  • Protein Techniques
  • Microscopy and Photography
  • Microscopy and Photomicrography Techniques.