The Nuclear Receptor Superfamily Methods and Protocols /
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| Format: | eBook |
| Language: | English |
| Published: |
Totowa, NJ :
Humana Press : Imprint: Humana,
2009.
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| Edition: | 1st ed. 2009. |
| Series: | Methods in Molecular Biology,
505 |
| Subjects: | |
| Online Access: | https://doi.org/10.1007/978-1-60327-575-0 |
Table of Contents:
- Nuclear Receptors: One Big Family
- Ligand Binding and Nuclear Receptor Turnover
- Methods for Measuring Ligand Dissociation and Nuclear Receptor Turnover in Whole Cells
- Flow Cytometry as a Tool for Measurement of Steroid Hormone Receptor Protein Expression in Leukocytes
- X-Ray Crystallography of Agonist/Antagonist-Bound Receptors
- Nuclear Localization and DNA Binding
- FRAP and FRET Methods to Study Nuclear Receptors in Living Cells
- Receptor-DNA Interactions: EMSA and Footprinting
- Chromatin Immunoprecipitation (ChIP) Methodology and Readouts
- Nuclear Receptor-Co-Regulatory Protein Interactions
- Yeast-Based Reporter Assays for the Functional Characterization of Cochaperone Interactions with Steroid Hormone Receptors
- High Throughput Analysis of Nuclear Receptor-Cofactor Interactions
- Binding Affinity and Kinetic Analysis of Nuclear Receptor/Co-Regulator Interactions Using Surface Plasmon Resonance
- Using RNA Interference to Study Protein Function
- Using Intrinsic Fluorescence Emission Spectroscopy to Study Steroid Receptor and Coactivator Protein Conformation Dynamics
- Pathophysiological Analysis of Nuclear Receptor Function
- Development of Phosphorylation Site-Specific Antibodies to Nuclear Receptors
- Tissue-Selective Knockouts of Steroid Receptors: A Novel Paradigm in the Study of Steroid Action
- Methods for Identifying and Studying Genetic Alterations in Hormone-Dependent Cancers.