Phospho-Proteomics Methods and Protocols /

Detalles Bibliográficos
Autor Corporativo: SpringerLink (Online service)
Otros Autores: de Graauw, Marjo. (Editor )
Formato: eBook
Lenguaje:English
Publicado: Totowa, NJ : Humana Press : Imprint: Humana, 2009.
Edición:1st ed. 2009.
Colección:Methods in Molecular Biology, 527
Materias:
Acceso en línea:https://doi.org/10.1007/978-1-60327-834-8
Tabla de Contenidos:
  • Protein Phosphorylation and 2D Gel Electrophoresis
  • A High-Resolution Two Dimensional Gel- and Pro-Q DPS-Based Proteomics Workflow for Phosphoprotein Identification and Quantitative Profiling
  • Differential Phosphoprotein Labelling (DIPPL) Using 32P and 33P
  • Enrichment of Phosphoproteins and Peptides
  • Identification of Oxidative Stress-Induced Tyrosine Phosphorylated Proteins by Immunoprecipitation and Mass Spectrometry
  • Enrichment and Characterization of Phosphopeptides by Immobilized Metal Affinity Chromatography (IMAC) and Mass Spectrometry
  • The Use of Titanium Dioxide Micro-Columns to Selectively Isolate Phosphopeptides from Proteolytic Digests
  • Enrichment and Separation of Mono- and Multiply Phosphorylated Peptides Using Sequential Elution from IMAC Prior to Mass Spectrometric Analysis
  • Use of Stable Isotope Labeling by Amino Acids in Cell Culture (SILAC) for Phosphotyrosine Protein Identification and Quantitation
  • Hydrophilic Interaction Chromatography for Fractionation and Enrichment of the Phosphoproteome
  • SILAC for Global Phosphoproteomic Analysis
  • Quantitative Phospho-proteomics Based on Soluble Nanopolymers
  • Profiling the Tyrosine Phosphorylation State Using SH2 Domains
  • Phosphoprotein Analysis by Mass Spectrometry
  • An Overview of the Qualitative Analysis of Phosphoproteins by Mass Spectrometry
  • The Analysis of Phosphoproteomes by Selective Labelling and Advanced Mass Spectrometric Techniques
  • On-Line Liquid Chromatography Electron Capture Dissociation for the Characterization of Phosphorylation Sites in Proteins
  • Quantification of Protein Phosphorylation by ?LC-ICP-MS
  • Reverse-Phase Diagonal Chromatography for Phosphoproteome Research
  • Chemical Tagging Strategies for Mass Spectrometry-Based Phospho-proteomics
  • Arrays to Study Protein-Phosphorylation
  • Antibody Array Platform to Monitor Protein Tyrosine Phosphorylation in Mammalian Cells
  • Protein Tyrosine Kinase Characterization Based on Fully Automated Synthesis of (Phospho) Peptide Arrays in Microplates
  • Kinome Profiling Using Peptide Arrays in Eukaryotic Cells
  • Bioinformatics
  • ProMoST: A Tool for Calculating the pI and Molecular Mass of Phosphorylated and Modified Proteins on Two-Dimensional Gels
  • Kinase-Specific Prediction of Protein Phosphorylation Sites
  • Reconstructing Regulatory Kinase Pathways from Phosphopeptide Data: A Bioinformatics Approach.