Zebrafish Methods and Protocols /

Detalles Bibliográficos
Autor Corporativo: SpringerLink (Online service)
Otros Autores: Lieschke, Graham J. (Editor ), Oates, Andrew C. (Editor ), Kawakami, Koichi. (Editor )
Formato: eBook
Lenguaje:English
Publicado: Totowa, NJ : Humana Press : Imprint: Humana, 2009.
Edición:1st ed. 2009.
Colección:Methods in Molecular Biology, 546
Materias:
Acceso en línea:https://doi.org/10.1007/978-1-60327-977-2
Tabla de Contenidos:
  • Mutagenesis and Mutants
  • Highly Efficient ENU Mutagenesis in Zebrafish
  • Production of Pseudotyped Retrovirus and the Generation of Proviral Transgenic Zebrafish
  • Undertaking a Successful Gynogenetic Haploid Screen in Zebrafish
  • Cryopreservation and In Vitro Fertilization at the Zebrafish International Resource Center
  • Transgenesis
  • Transient and Stable Transgenesis Using Tol2 Transposon Vectors
  • Analysis of Genes and Genome by the Tol2-Mediated Gene and Enhancer Trap Methods
  • Bacterial Artificial Chromosome Transgenesis for Zebrafish
  • Simple and Efficient Transgenesis with Meganuclease Constructs in Zebrafish
  • Tissue-Specific Manipulations
  • Nitroreductase-Mediated Cell Ablation in Transgenic Zebrafish Embryos
  • Focal Electroporation in Zebrafish Embryos and Larvae
  • Tissue Micromanipulation in Zebrafish Embryos
  • Analyzing Gene Expression
  • Zebrafish Spotted-Microarray for Genome-Wide Expression Profiling Experiments. Part I: Array Printing and Hybridization
  • Zebrafish Spotted-Microarray for Genome-Wide Expression Profiling Experiments: Data Acquisition and Analysis
  • Validating microRNA Target Transcripts Using Zebrafish Assays
  • Imaging
  • Multiple Embryo Time-Lapse Imaging of Zebrafish Development
  • Live Cell Imaging of Zebrafish Leukocytes
  • Imaging Zebrafish Embryos by Two-Photon Excitation Time-Lapse Microscopy
  • Morphological Analysis of the Zebrafish Digestive System
  • In Toto Imaging of Embryogenesis with Confocal Time-Lapse Microscopy.