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03005nam a22003615i 4500 |
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978-1-60327-993-2 |
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20191027051639.0 |
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cr nn 008mamaa |
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100301s2009 xxu| s |||| 0|eng d |
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|a 9781603279932
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7 |
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|a 10.1007/978-1-60327-993-2
|2 doi
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| 040 |
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|a Sistema de Bibliotecas del Tecnológico de Costa Rica
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| 245 |
1 |
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|a Mitosis
|b Methods and Protocols /
|c edited by Andrew D. McAinsh.
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| 250 |
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|a 1st ed. 2009.
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| 260 |
# |
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|a Totowa, NJ :
|b Humana Press :
|b Imprint: Humana,
|c 2009.
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| 300 |
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|a XI, 345 p.
|b online resource.
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| 336 |
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|a text
|b txt
|2 rdacontent
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| 337 |
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|a computer
|b c
|2 rdamedia
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| 338 |
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|a online resource
|b cr
|2 rdacarrier
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| 490 |
1 |
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|a Methods in Molecular Biology,
|v 545
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| 505 |
0 |
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|a Knock-in and Knock-out: The Use of Reverse Genetics in Somatic Cells to Dissect Mitotic Pathways -- Functional Dissection of Mitotic Regulators Through Gene Targeting in Human Somatic Cells -- RNAi in Drosophila S2 Cells as a Tool for Studying Cell Cycle Progression -- Production of Mitotic Regulators Using an Autoselection System for Protein Expression in Budding Yeast -- Hydrodynamic Analysis of Human Kinetochore Complexes During Mitosis -- Isolation of Protein Complexes Involved in Mitosis and Cytokinesis from Drosophila Cultured Cells -- Automated Live Microscopy to Study Mitotic Gene Function in Fluorescent Reporter Cell Lines -- Electron Tomography of Microtubule End-Morphologies in C. elegans Embryos -- Dissecting Mitosis with Laser Microsurgery and RNAi in Drosophila Cells -- Fluorescence Imaging of the Centrosome Cycle in Mammalian Cells -- Visualization of Fluorescence-Tagged Proteins in Fission Yeast: The Analysis of Mitotic Spindle Dynamics Using GFP-Tubulin Under the Native Promoter -- Analysing Kinetochore Function in Human Cells: Spindle Checkpoint and Chromosome Congression -- Probing Kinetochore Structure and Function Using Xenopus laevis Frog Egg Extracts -- Live Cell Imaging of Kinetochore Capture by Microtubules in Budding Yeast -- The Spindle Checkpoint: Assays for the Analysis of Spindle Checkpoint Arrest and Recovery -- Measuring Proteolysis in Mitosis -- An In Vitro Assay for Cdc20-Dependent Mitotic Anaphase-Promoting Complex Activity from Budding Yeast -- In Vitro Assays for the Anaphase-Promoting Complex/Cyclosome (APC/C) in Xenopus Egg Extracts -- Preparation of Synchronized Human Cell Extracts to Study Ubiquitination and Degradation -- Biochemical Analysis of the Anaphase Promoting Complex: Activities of E2 Enzymes and Substrate Competitive (Pseudosubstrate) Inhibitors.
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| 650 |
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|a Cell biology.
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| 650 |
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|a Developmental biology.
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| 650 |
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|a Cell cycle.
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| 650 |
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|a Gene expression.
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| 650 |
1 |
4 |
|a Cell Biology.
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| 650 |
2 |
4 |
|a Developmental Biology.
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| 650 |
2 |
4 |
|a Cell Cycle Analysis.
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| 650 |
2 |
4 |
|a Gene Expression.
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| 700 |
1 |
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|a McAinsh, Andrew D.
|e editor.
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| 710 |
2 |
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|a SpringerLink (Online service)
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| 773 |
0 |
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|t Springer eBooks
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| 856 |
4 |
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|u https://doi.org/10.1007/978-1-60327-993-2
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