Mitosis Methods and Protocols /
| Autor Corporativo: | |
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| Otros Autores: | |
| Formato: | eBook |
| Lenguaje: | English |
| Publicado: |
Totowa, NJ :
Humana Press : Imprint: Humana,
2009.
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| Edición: | 1st ed. 2009. |
| Colección: | Methods in Molecular Biology,
545 |
| Materias: | |
| Acceso en línea: | https://doi.org/10.1007/978-1-60327-993-2 |
Tabla de Contenidos:
- Knock-in and Knock-out: The Use of Reverse Genetics in Somatic Cells to Dissect Mitotic Pathways
- Functional Dissection of Mitotic Regulators Through Gene Targeting in Human Somatic Cells
- RNAi in Drosophila S2 Cells as a Tool for Studying Cell Cycle Progression
- Production of Mitotic Regulators Using an Autoselection System for Protein Expression in Budding Yeast
- Hydrodynamic Analysis of Human Kinetochore Complexes During Mitosis
- Isolation of Protein Complexes Involved in Mitosis and Cytokinesis from Drosophila Cultured Cells
- Automated Live Microscopy to Study Mitotic Gene Function in Fluorescent Reporter Cell Lines
- Electron Tomography of Microtubule End-Morphologies in C. elegans Embryos
- Dissecting Mitosis with Laser Microsurgery and RNAi in Drosophila Cells
- Fluorescence Imaging of the Centrosome Cycle in Mammalian Cells
- Visualization of Fluorescence-Tagged Proteins in Fission Yeast: The Analysis of Mitotic Spindle Dynamics Using GFP-Tubulin Under the Native Promoter
- Analysing Kinetochore Function in Human Cells: Spindle Checkpoint and Chromosome Congression
- Probing Kinetochore Structure and Function Using Xenopus laevis Frog Egg Extracts
- Live Cell Imaging of Kinetochore Capture by Microtubules in Budding Yeast
- The Spindle Checkpoint: Assays for the Analysis of Spindle Checkpoint Arrest and Recovery
- Measuring Proteolysis in Mitosis
- An In Vitro Assay for Cdc20-Dependent Mitotic Anaphase-Promoting Complex Activity from Budding Yeast
- In Vitro Assays for the Anaphase-Promoting Complex/Cyclosome (APC/C) in Xenopus Egg Extracts
- Preparation of Synchronized Human Cell Extracts to Study Ubiquitination and Degradation
- Biochemical Analysis of the Anaphase Promoting Complex: Activities of E2 Enzymes and Substrate Competitive (Pseudosubstrate) Inhibitors.