Low-Cost Methods for Molecular Characterization of Mutant Plants Tissue Desiccation, DNA Extraction and Mutation Discovery: Protocols /

Low-Cost Methods for Molecular Characterization of Mutant Plants Tissue Desiccation, DNA Extraction and Mutation Discovery: Protocols /

Detalles Bibliográficos
Autores principales: Till, Bradley J. (Autor), Jankowicz-Cieslak, Joanna. (Autor), Huynh, Owen A. (Autor), Beshir, Mayada M. (Autor), Laport, Robert G. (Autor), Hofinger, Bernhard J. (Autor)
Autor Corporativo: SpringerLink (Online service)
Formato: eBook
Lenguaje:English
Publicado: Cham : Springer International Publishing : Imprint: Springer, 2015.
Edición:1st ed. 2015.
Materias:
Plant breeding.
Biology—Technique.
Nucleic acids.
Plant Breeding/Biotechnology.
Biological Techniques.
Nucleic Acid Chemistry.
Acceso en línea:https://doi.org/10.1007/978-3-319-16259-1
Tabla de Contenidos:
  • Introduction
  • Health and Safety Considerations
  • Sample Collection and Storage
  • Low-Cost DNA Extraction
  • PCR Amplification for Low-Cost Mutation Discovery
  • Enzymatic Mismatch Cleavage and Agarose Gel Evaluation of Samples
  • Alternative Enzymology for Mismatch Cleavage for TILLING and Ecotilling: Extraction of Enzymes form Common Weedy Plants
  • Example Data
  • Conclusions.  .

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